The interaction between β-catenin, GSK3β and APC after motogen induced cell-cell dissociation, and their involvement in signal transduction pathways in prostate cancer

  • Authors:
    • G. Davies
    • W. G. Jiang
    • M. D. Mason
  • Corresponding author:
  • View Affiliations

  • Published online on: Sunday, April 1, 2001
  • Pages: 843-847 DOI: 10.3892/ijo.18.4.843

Abstract

The effect of HGF/SF was examined on the interactions between APC, GSK3β and β-catenin in prostate cancer cells LNCapFGC (E-cadherin positive) and PC-3 (E-cadherin negative). Using immunoprecipitation, APC was found to be co-precipitated with either GSK3β or β-catenin in both cell lines. Stimulation with HGF/SF showed no change in the co-precipitation status of these protein molecules. In contrast, co-precipitation between GSK3β and β-catenin was only observed in LNCapFGC cells, and increased upon continued exposure to the motogen HGF/SF. Furthermore, using immunofluorescence, stimulation with HGF/SF was found to increase the level of co-localised cytoplasmic staining between β-catenin and GSK3β, in prostate cancer cells. RT-PCR revealed that there were no mutations within the binding regions between β-catenin and GSK3β. It is concluded, that uncomplexed cytoplasmic pools of β-catenin associate more readily with the Axin complex in the absence of E-cadherin. Whereas, in the presence of E-cadherin, β-catenin is stabilised by forming tight cell-cell contacts which may influence the invasive potential of cancer cells.
Journal Cover

April 2001
Volume 18 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2014 Impact Factor: 3.025
Ranked #30/211 Oncology
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APA
Davies, G., Jiang, W.G., & Mason, M.D. (2001). The interaction between β-catenin, GSK3β and APC after motogen induced cell-cell dissociation, and their involvement in signal transduction pathways in prostate cancer. International Journal of Oncology, 18, 843-847. http://dx.doi.org/10.3892/ijo.18.4.843
MLA
Davies, G., Jiang, W. G., Mason, M. D."The interaction between β-catenin, GSK3β and APC after motogen induced cell-cell dissociation, and their involvement in signal transduction pathways in prostate cancer". International Journal of Oncology 18.4 (2001): 843-847.
Chicago
Davies, G., Jiang, W. G., Mason, M. D."The interaction between β-catenin, GSK3β and APC after motogen induced cell-cell dissociation, and their involvement in signal transduction pathways in prostate cancer". International Journal of Oncology 18, no. 4 (2001): 843-847. http://dx.doi.org/10.3892/ijo.18.4.843