Predictive prognostic role of miR‑181a with discrepancy in the liver and serum of genotype 4 hepatitis C virus patients

  • Authors:
    • Dalia Sherif Elhelw
    • Radwa Yehia Mekky
    • Nada El‑Ekiaby
    • Rasha Ahmed
    • Mohammad Ahmed Mohey Eldin
    • Mohammad El‑Sayed
    • Mahmoud Mohammad Abouelkhair
    • Ayman Salah
    • Abdel Rahman Zekri
    • Gamal Esmat
    • Ahmed Ihab Abdelaziz
  • View Affiliations

  • Published online on: August 13, 2014     https://doi.org/10.3892/br.2014.343
  • Pages: 843-848
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Abstract

microRNA (miRNA) expression in organs does not always represent their quantity in serum. A disparity in the expression of miR‑181a has been reported in the tissues and serum of hepatocellular carcinoma (HCC) patients. Since hepatitis C virus (HCV) is a major cause of HCC and miR‑181a has never been studied in HCV, the present study aimed to investigate the miR‑181a expression profile in genotype 4 (GT4)‑HCV patients to evaluate whether this pattern is also apparent in HCV. RNA was extracted from liver tissues, peripheral mononuclear cells (PBMCs) and serum samples from GT4‑HCV‑infected patients and healthy donors to evaluate the relative miR‑181a expression using quantitative reverse transcription‑polymerase chain reaction. miR‑181a was significantly higher in the serum of naïve patients compared to controls, and an inverse correlation with the viral load and liver enzymes was apparent. By contrast, no difference in miR‑181a expression was observed in the liver tissues and PBMCs of patients compared to controls. This expression observed in HCV is conflicting to that previously reported in HCC. The study also demonstrates a significant upregulation of miR‑181a post‑interferon/ribavirin treatment in the serum of sustained virological responders (SVRs) compared to non‑responders and treatment‑naïve SVRs. In conclusion, miR‑181a may be considered to be a possible prognostic marker in GT4‑HCV infection.
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November-December 2014
Volume 2 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Elhelw DS, Mekky RY, El‑Ekiaby N, Ahmed R, Mohey Eldin MA, El‑Sayed M, Abouelkhair MM, Salah A, Zekri AR, Esmat G, Esmat G, et al: Predictive prognostic role of miR‑181a with discrepancy in the liver and serum of genotype 4 hepatitis C virus patients. Biomed Rep 2: 843-848, 2014
APA
Elhelw, D.S., Mekky, R.Y., El‑Ekiaby, N., Ahmed, R., Mohey Eldin, M.A., El‑Sayed, M. ... Abdelaziz, A.I. (2014). Predictive prognostic role of miR‑181a with discrepancy in the liver and serum of genotype 4 hepatitis C virus patients. Biomedical Reports, 2, 843-848. https://doi.org/10.3892/br.2014.343
MLA
Elhelw, D. S., Mekky, R. Y., El‑Ekiaby, N., Ahmed, R., Mohey Eldin, M. A., El‑Sayed, M., Abouelkhair, M. M., Salah, A., Zekri, A. R., Esmat, G., Abdelaziz, A. I."Predictive prognostic role of miR‑181a with discrepancy in the liver and serum of genotype 4 hepatitis C virus patients". Biomedical Reports 2.6 (2014): 843-848.
Chicago
Elhelw, D. S., Mekky, R. Y., El‑Ekiaby, N., Ahmed, R., Mohey Eldin, M. A., El‑Sayed, M., Abouelkhair, M. M., Salah, A., Zekri, A. R., Esmat, G., Abdelaziz, A. I."Predictive prognostic role of miR‑181a with discrepancy in the liver and serum of genotype 4 hepatitis C virus patients". Biomedical Reports 2, no. 6 (2014): 843-848. https://doi.org/10.3892/br.2014.343