Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors

  • Authors:
    • Hironori Yoshino
    • Takahiro Saitoh
    • Masataka Kozakai
    • Ikuo Kashiwakura
  • View Affiliations

  • Published online on: November 7, 2014     https://doi.org/10.3892/br.2014.377
  • Pages: 59-62
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Abstract

Retinoic acid‑inducible gene‑I (RIG‑I)‑like receptors [RLRs; RIG‑I and melanoma differentiation‑associated gene 5 (MDA5)] sense virus‑derived RNA or a synthetic analog of double‑stranded RNA polyinosinic‑polycytidylic acid [poly(I:C)] and are responsible for host defense against viruses. However, it remains unclear whether radiation affects RLRs. Therefore, the present study investigated the effects of ionizing radiation on RIG‑I and MDA5 expression and the response to poly(I:C) using THP1 (human monocytic cell line)‑derived macrophages. Non‑ and X‑irradiated (1‑10 Gy) macrophages expressed RIG‑I and MDA5 at mRNA and protein levels and there was no significant difference in the expression levels. Non‑ and X‑irradiated macrophages expressed antiviral cytokine interferon (IFN)‑β mRNA following poly(I:C)‑low molecular weight/LyoVec™ and poly(I:C)‑high molecular weight/LyoVec™ stimulation, the agonist of RIG‑I and MDA5, respectively. In line with the results of the expression of RIG‑I and MDA5, no significant difference in the expression of IFN‑β mRNA was observed between non‑ and X‑irradiation. These results indicate that ionizing radiation hardly affects RLR expression and the response to their agonist poly(I:C) in THP1‑derived macrophages.
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January-February 2015
Volume 3 Issue 1

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Spandidos Publications style
Yoshino H, Saitoh T, Kozakai M and Kashiwakura I: Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors. Biomed Rep 3: 59-62, 2015
APA
Yoshino, H., Saitoh, T., Kozakai, M., & Kashiwakura, I. (2015). Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors. Biomedical Reports, 3, 59-62. https://doi.org/10.3892/br.2014.377
MLA
Yoshino, H., Saitoh, T., Kozakai, M., Kashiwakura, I."Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors". Biomedical Reports 3.1 (2015): 59-62.
Chicago
Yoshino, H., Saitoh, T., Kozakai, M., Kashiwakura, I."Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors". Biomedical Reports 3, no. 1 (2015): 59-62. https://doi.org/10.3892/br.2014.377