Response of HT29 cells to butyrate depends on time of exposure and glucose deprivation

  • Authors: Dana Kucerová, Jitka Stokrová, Jan Korb, Eva Sloncová, Zdena Tuhácková, Vlasta Sovová
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  • Published online on: Sunday, December 1, 2002
  • Pages: 779-784
  • DOI: 10.3892/ijmm.10.6.779

Abstract

The high level of alkaline phosphatase activity in HT29 cells induced after 2 or 5 days of butyrate treatment was decreased during their prolonged exposure (about 30 days) to this agent together with a decrease of sensitivity to apoptosis. However, an enormous additive effect on alkaline phosphatase activity was found after butyrate treatment of glucose-starved cells. In concert with this finding, the substructural analysis revealed a dense brush border, tendency to polarization and morphologically normal mitochondria. It can be concluded that prolonged butyrate treatment of HT29 cells attenuated their response to this agent. On the other hand, glucose deprivation, as another inductor of differentiation, was found to increase the sensitivity of HT29 cells to butyrate.
Journal Cover

December 2002
Volume 10 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
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APA
Kucerová, D., Stokrová, J., Korb, J., Sloncová, E., Tuhácková, Z., & Sovová, V. (2002). Response of HT29 cells to butyrate depends on time of exposure and glucose deprivation. International Journal of Molecular Medicine, 10(6), 779-784.
MLA
Kucerová, Stokrová, Korb, Sloncová, Tuhácková, and Vlasta Sovová. "Response of HT29 cells to butyrate depends on time of exposure and glucose deprivation." International Journal of Molecular Medicine International Journal of Molecular Medicine 10.6 (2002): 779-784.
Chicago
Kucerová, Stokrová, Korb, Sloncová, Tuhácková, and Vlasta Sovová. "Response of HT29 cells to butyrate depends on time of exposure and glucose deprivation." International Journal of Molecular Medicine International Journal of Molecular Medicine 10 no. 6 (2002): 779-784.