Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells

  • Authors: Yasuhiro Ogawa, Toshihiro Kobayashi, Akihito Nishioka, Shinji Kariya, Shinji Hamasato, Harumichi Seguchi, Shoji Yoshida
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  • Published online on: Wednesday, January 1, 2003
  • Pages: 27-32
  • DOI: 10.3892/ijmm.11.1.27

Abstract

The mechanism leading to the high level of radiosensitivity of T lymphocytes has not yet been fully described. In our previous study, we demonstrated that human peripheral T lymphocytes revealed early apoptotic changes (annexin V-positive) and late apoptotic changes (propidium iodide-positive), at 13 and 24 h after irradiation of 5 Gy, respectively. Changes in mitochondrial membrane potential were observed at 10 h after irradiation of 5 Gy. Subsequently, mitochondrial cytochrome c release was confirmed. In order to elucidate the mechanism which occurs prior to the mitochondrial membrane potential changes, we examined in the present study the radiation dose and the timing of oxidative DNA damage induced in human peripheral T lymphocytes following 10 MV X-ray irradiation. As a result, the production of 8-oxoguanine, i.e., the product of oxidative DNA damage, was clearly identified starting at 10, 6, and 3 h, after 2, 5, and 20 Gy of irradiation, respectively. Therefore, we concluded that it remains necessary to evaluate the extent of radiation-induced oxidative DNA damage. Furthermore, it is important to analyze superoxide radical production and scavenging in terms of the variety of radiosensitivities found among various types of normal tissue cells and neoplastic cells.
Journal Cover

January 2003
Volume 11 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
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APA
Ogawa, Y., Kobayashi, T., Nishioka, A., Kariya, S., Hamasato, S., Seguchi, H., & Yoshida, S. (2003). Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells. International Journal of Molecular Medicine, 11(1), 27-32.
MLA
Ogawa, Kobayashi, Nishioka, Kariya, Hamasato, Seguchi, and Shoji Yoshida. "Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 11.1 (2003): 27-32.
Chicago
Ogawa, Kobayashi, Nishioka, Kariya, Hamasato, Seguchi, and Shoji Yoshida. "Radiation-induced oxidative DNA damage, 8-oxoguanine, in human peripheral T cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 11 no. 1 (2003): 27-32.