Superoxide anion is involved in the early apoptosis mediated by Gleditsia sinensis fruit extract

  • Authors: Ivy Tuang Ngo Teo, Johnny Cheuk On Tang, Chung Hin Chui, Gregory Yin Ming Cheng, Mabel Yin Chun Yau, Fung Yi Lau, Raymond Siu Ming Wong, Thomas Wai Tong Leung, Filly Cheung, Kwok Ping Ho, Chor Hing Cheng, Albert Sun Chi Chan
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  • Published online on: Tuesday, June 1, 2004
  • Pages: 909-913
  • DOI: 10.3892/ijmm.13.6.909

Abstract

Changes in the intracellular level of reactive oxygen species (ROS) including superoxide anion, hydroxyl radical, hydrogen peroxide and finally cellular acid-base equilibrium are reported to play an important role in the early step of apoptosis. All of which would precede the loss of mitochondrial membrane potential and releasing of those apoptotic inducing factors such as cytochrome c as well as caspases activation. Any potential chemotherapeutic agent that could drive such changes in ROS would be particularly attractive. Recently we have reported the potential use of Gleditsia sinensis extract (GSE) in cancer therapy including solid tumour and leukaemia cell lines as well as primary cultured leukaemia cells in vitro. We demonstrated that apoptotic activity is involved. Here we further showed that the mechanism of GSE induced apoptosis, including an early decreasing of intracellular superoxide anion as measured by nitroblue tetrazolium (NBT) reduction assay. This phenomenon readily occurred before any shrinkage of cancer cells including MDA-MB231 breast cancer, CNE-2 nasopharyngeal carcinoma, K-562 chronic myelogenous leukaemia and KG1-a, acute myelogenous leukaemia. Cell viability was determined by morphological investigation and the [3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) assay. Furthermore, the superoxide dismutase activity from those cellular extracts after GSE treatment seemed to be increased. Taken together, we speculate that the GSE-induced apoptosis, via ROS pathway, involves an early decrease of intracellular superoxide anion.
Journal Cover

June 2004
Volume 13 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Teo, I., Tang, J., Chui, C., Cheng, G., Yau, M., Lau, F., Wong, R., Leung, T., Cheung, F., Ho, K., Cheng, C., & Chan, A. (2004). Superoxide anion is involved in the early apoptosis mediated by Gleditsia sinensis fruit extract. International Journal of Molecular Medicine, 13(6), 909-913.
MLA
Teo, Tang, Chui, Cheng, Yau, Lau, Wong, Leung, Cheung, Ho, Cheng, and Albert Chan. "Superoxide anion is involved in the early apoptosis mediated by Gleditsia sinensis fruit extract." International Journal of Molecular Medicine International Journal of Molecular Medicine 13.6 (2004): 909-913.
Chicago
Teo, Tang, Chui, Cheng, Yau, Lau, Wong, Leung, Cheung, Ho, Cheng, and Albert Chan. "Superoxide anion is involved in the early apoptosis mediated by Gleditsia sinensis fruit extract." International Journal of Molecular Medicine International Journal of Molecular Medicine 13 no. 6 (2004): 909-913.