Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.

  • Authors:
    • Toshio Machida
    • Motoo Kubota
    • Naokatsu Saeki
    • Masaki Takiguchi
    • Akira Yamaura
    • Takaki Hiwasa
  • View Affiliations

  • Published online on: Friday, October 1, 2004
  • Pages: 659-721
  • DOI: 10.3892/ijmm.14.4.659

Abstract

Cigarette smoking is known to be one of the risk factors of subarachnoid hemorrhage (SAH), yet the precise mechanism remains to be proved. Based on the hypothesis that smoke components might enhance protease activity in the arterial wall, we examined the proteinase activities of plasma from smoke-extract-injected rats. Rats were injected intraperitoneally with smoke extract and the protease activity in the plasma was examined by zymography in the presence or the absence of proteinase inhibitors. Metalloproteinase activity was measured using a synthetic substrate. Proteolytic activities of 80- and 100-kDa against gelatin and collagen type I-V were induced 6 h after the injection. Inhibition of the activity by a metalloproteinase inhibitor but not by serine, cysteine or aspartic protease inhibitors suggested that the proteinases can be attributed to metalloproteinases. Cleavage activity in plasma toward a synthetic metalloproteinase substrate also increased within 24 h after the injection of the smoke extract. The results indicate that the induction of plasma metalloproteinase activity by smoke extract might account for the causal role of smoking in the development of SAH.
Journal Cover

October 2004
Volume 14 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Machida, T., Kubota, M., Saeki, N., Takiguchi, M., Yamaura, A., & Hiwasa, T. (2004). Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.. International Journal of Molecular Medicine, 14(4), 659-721.
MLA
Machida, Kubota, Saeki, Takiguchi, Yamaura, and Takaki Hiwasa. "Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.." International Journal of Molecular Medicine International Journal of Molecular Medicine 14.4 (2004): 659-721.
Chicago
Machida, Kubota, Saeki, Takiguchi, Yamaura, and Takaki Hiwasa. "Increase in metalloproteinase activity in the plasma of smoke-extract-injected rats.." International Journal of Molecular Medicine International Journal of Molecular Medicine 14 no. 4 (2004): 659-721.