Does the PGC-1/PPARγ pathway play a role in Com-1/p8 mediated cell growth inhibition in prostate cancer?

  • Authors:
    • Wen G. Jiang
    • Gaynor Davies
    • Howard Kynaston
    • Malcolm D. Mason
    • Oystein Fodstad
  • View Affiliations

  • Published online on: December 1, 2006     https://doi.org/10.3892/ijmm.18.6.1169
  • Pages: 1169-1175
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Abstract

Com-1, candidate of metastasis-1, also known as p8, has been shown to regulate the growth and apoptosis of cancer cells and is associated with the disease progression in human cancers including prostate cancer. In the current study, we investigated a potential mechanism underlying the anticancer action of Com-1/p8 in human prostate cancer. Human prostate cancer cells were used. Full-length Com-1 cDNA was isolated from normal mammary tissues. Ribozyme transgenes that specifically target human Com-1 were constructed using the pEF6/V5-His vector. Com-1 interacting proteins were determined using immunoprecipitation method. Cell growth and invasiveness were investigated using in vitro methods. Using immunoprecipitation and Western blotting, Com-1 was found to be cross-reprecipitated with PGC-1, a coactivator of peroxisome proliferator activated receptor (PPAR)-γ, but not PPAR-γ itself. Elimination of Com-1 from prostate cancer cells resulted in a reduced response of the cells to ciglitizone, a PPAR-γ agonist, whereas forced expression of Com-1 rendered cells more responsive to ciglitizone. We further demonstrated that the overexpression of Com-1/p8 resulted in changes in the expression of the PGC-1 responsive gene, fatty acid synthase (FAS). Com-1 may act as a tumour suppressor in human prostate cancer cells. The potential tumour suppressive effect of Com-1 is at least partly via its interaction with PGC-1, the PPAR-γ coactivator.

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December 2006
Volume 18 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jiang WG, Davies G, Kynaston H, Mason MD and Fodstad O: Does the PGC-1/PPARγ pathway play a role in Com-1/p8 mediated cell growth inhibition in prostate cancer?. Int J Mol Med 18: 1169-1175, 2006
APA
Jiang, W.G., Davies, G., Kynaston, H., Mason, M.D., & Fodstad, O. (2006). Does the PGC-1/PPARγ pathway play a role in Com-1/p8 mediated cell growth inhibition in prostate cancer?. International Journal of Molecular Medicine, 18, 1169-1175. https://doi.org/10.3892/ijmm.18.6.1169
MLA
Jiang, W. G., Davies, G., Kynaston, H., Mason, M. D., Fodstad, O."Does the PGC-1/PPARγ pathway play a role in Com-1/p8 mediated cell growth inhibition in prostate cancer?". International Journal of Molecular Medicine 18.6 (2006): 1169-1175.
Chicago
Jiang, W. G., Davies, G., Kynaston, H., Mason, M. D., Fodstad, O."Does the PGC-1/PPARγ pathway play a role in Com-1/p8 mediated cell growth inhibition in prostate cancer?". International Journal of Molecular Medicine 18, no. 6 (2006): 1169-1175. https://doi.org/10.3892/ijmm.18.6.1169