Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle

  • Authors:
    • Kathleen O'Connell
    • Joan Gannon
    • Philip Doran
    • Kay Ohlendieck
  • View Affiliations

  • Published online on: August 1, 2007     https://doi.org/10.3892/ijmm.20.2.145
  • Pages: 145-153
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Abstract

Extended longevity is often accompanied by frailty and increased susceptibility to a variety of crippling disorders. One of the most striking features of human aging is sarcopenia, which is defined as the age-related decline in skeletal muscle mass and strength. Although various metabolic and functional defects in aging muscle fibres have been described over the last decade, it is not known whether a pathophysiological hierarchy exists within degenerative pathways leading to muscle wasting. Hence, in order to identify novel biomarkers of age-dependent skeletal muscle degeneration, we have here applied mass spectrometry-based proteomics for studying global muscle protein expression patterns. As a model system of sarcopenia, we have employed crude extracts from senescent rat gastrocnemius muscle, as compared to young adult tissue preparations. Using the highly sensitive protein dye Deep Purple for the analysis of the 2-D separated muscle proteome and peptide mass fingerprinting for the identification of individual protein spots, a differential expression pattern was observed for contractile proteins, metabolic factors, regulatory components and heat shock elements. A drastic increase was shown for αB-crystallin, myosin light chain MLC-1, phosphoglycerate kinase, adenylate kinase, triosephosphate isomerase, albumin, aconitase and nucleoside-diphosphate kinase in aged fibres. In contrast, the expression of pyruvate kinase, aldolase, creatine kinase, transferrin, α-tropomyosin and myosin light chain MLC-3 was decreased in old skeletal muscle. Comparative 2-D immunoblotting of selected candidate proteins has confirmed the effect of aging on the skeletal muscle proteome. These findings demonstrate a severely perturbed protein expression pattern in aged skeletal muscle, which reflects the underlying molecular alterations causing a drastic decline of muscle strength in the senescent organism. In the long-term, the systematic deduction of abnormal protein expression in aged muscle by proteomic profiling approaches may lead to the cataloguing of a cohort of novel therapeutic targets to treat muscular weakness in the aging population.

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August 2007
Volume 20 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
O'Connell K, Gannon J, Doran P and Ohlendieck K: Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle. Int J Mol Med 20: 145-153, 2007
APA
O'Connell, K., Gannon, J., Doran, P., & Ohlendieck, K. (2007). Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle. International Journal of Molecular Medicine, 20, 145-153. https://doi.org/10.3892/ijmm.20.2.145
MLA
O'Connell, K., Gannon, J., Doran, P., Ohlendieck, K."Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle". International Journal of Molecular Medicine 20.2 (2007): 145-153.
Chicago
O'Connell, K., Gannon, J., Doran, P., Ohlendieck, K."Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle". International Journal of Molecular Medicine 20, no. 2 (2007): 145-153. https://doi.org/10.3892/ijmm.20.2.145