Open Access

Sema4A induces cell morphological changes through B-type plexin-mediated signaling

  • Authors:
    • Kazunori Yukawa
    • Tetsuji Tanaka
    • Kenji Yoshida
    • Noriko Takeuchi
    • Takuji Ito
    • Hyota Takamatsu
    • Hitoshi Kikutani
    • Atsushi Kumanogoh
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/ijmm_00000334
  • Pages: 225-230
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Abstract

Semaphorins are a family of secreted and membrane-bound proteins known as axonal pathfinders. Sema4A, a member of class 4 semaphorins, induces growth cone collapse of hippocampal neurons. The binding of Sema4A to growth cones indicates the presence of receptors transmitting signals through the intracellular effectors to induce growth cone collapse in hippocampal neurons. Transfection experiments of the candidate receptor genes into COS-7 cells demonstrated that Sema4A binds to axonal guidance receptors Plexin-B1, -B2 and -B3. To identify the functional Sema4A receptor and the signal transduction machinery, COS-7 cell contraction assay was performed, in which intracellular signal transmission induced by Sema4A triggered cell contraction. Expression vectors encoding plexins and Rnd1, a Rho family GTPase, were transfected into COS-7 cells, and a proportion of contracted cells among the transfectants was determined after incubation with Sema4A. The results demonstrated that the combination of Rnd1 and Plexin-B1, -B2 or -B3 induced significant cell contraction, indicating that B-type plexins transmit an intracellular signal of Sema4A through Rnd1. To further study the mechanism of B-type plexin-mediated signaling in Sema4A-induced growth cone collapse, mouse hippocampal neurons transfected with a control or expression plasmid encoding a constitutively active mutant of R-Ras (R-RasQL) were stimulated with Sema4A, followed by the assessment of growth cone collapse. Expression of R-RasQL significantly blocked Sema4A-induced growth cone collapse in the hippocampal neurons compared with the control plasmid. Sema4A thus induces growth cone collapse through the down-regulation of R-Ras activity in mouse hippocampal neurons.

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February 2010
Volume 25 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yukawa K, Tanaka T, Yoshida K, Takeuchi N, Ito T, Takamatsu H, Kikutani H and Kumanogoh A: Sema4A induces cell morphological changes through B-type plexin-mediated signaling. Int J Mol Med 25: 225-230, 2010
APA
Yukawa, K., Tanaka, T., Yoshida, K., Takeuchi, N., Ito, T., Takamatsu, H. ... Kumanogoh, A. (2010). Sema4A induces cell morphological changes through B-type plexin-mediated signaling. International Journal of Molecular Medicine, 25, 225-230. https://doi.org/10.3892/ijmm_00000334
MLA
Yukawa, K., Tanaka, T., Yoshida, K., Takeuchi, N., Ito, T., Takamatsu, H., Kikutani, H., Kumanogoh, A."Sema4A induces cell morphological changes through B-type plexin-mediated signaling". International Journal of Molecular Medicine 25.2 (2010): 225-230.
Chicago
Yukawa, K., Tanaka, T., Yoshida, K., Takeuchi, N., Ito, T., Takamatsu, H., Kikutani, H., Kumanogoh, A."Sema4A induces cell morphological changes through B-type plexin-mediated signaling". International Journal of Molecular Medicine 25, no. 2 (2010): 225-230. https://doi.org/10.3892/ijmm_00000334