Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis

  • Authors:
    • Liang Peng
    • Xiuli Men
    • Wenjian Zhang
    • Haiyan Wang
    • Shiqing  Xu
    • Mei Xu
    • Yaping Xu
    • Wenying Yang
    • Jinning Lou
  • View Affiliations

  • Published online on: April 28, 2011     https://doi.org/10.3892/ijmm.2011.684
  • Pages: 161-169
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Abstract

Pancreatic β-cell dysfunction is a critical component in the pathogenesis of diabetes. Endoplasmic reticulum (ER) stress is one of the factors that induces pancreatic β-cell dysfunction, but the underlying mechanisms have not been well elucidated. In this study, we report that a mitochondrial fission modulator, dynamin-related protein 1 (DRP-1), plays an important role in ER stress-induced β-cell apoptosis. Induction of DRP-1 expression significantly promoted ER stress-induced apoptosis in the DRP-1 WT (DRP-1 wild- type) inducible β-cell line, but not in the DRP-1 K38A (a dominant negative mutant of DRP-1) inducible β-cell line. We further demonstrated that the mitochondrial membrane potential decreased, and that cytochrome c release, caspase-3 activation and generation of reactive oxygen species (ROS) were enhanced by induction of DRP-1 WT, but prevented by DRP-1 K38A in pancreatic β-cells under ER stress conditions. These results indicate that DRP-1 mediates ER stress-induced pancreatic β-cell apoptosis.

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August 2011
Volume 28 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Peng L, Men X, Zhang W, Wang H, Xu S, Xu M, Xu Y, Yang W and Lou J: Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis. Int J Mol Med 28: 161-169, 2011
APA
Peng, L., Men, X., Zhang, W., Wang, H., Xu, S., Xu, M. ... Lou, J. (2011). Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis. International Journal of Molecular Medicine, 28, 161-169. https://doi.org/10.3892/ijmm.2011.684
MLA
Peng, L., Men, X., Zhang, W., Wang, H., Xu, S., Xu, M., Xu, Y., Yang, W., Lou, J."Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis". International Journal of Molecular Medicine 28.2 (2011): 161-169.
Chicago
Peng, L., Men, X., Zhang, W., Wang, H., Xu, S., Xu, M., Xu, Y., Yang, W., Lou, J."Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis". International Journal of Molecular Medicine 28, no. 2 (2011): 161-169. https://doi.org/10.3892/ijmm.2011.684