Knockdown of TRAF4 expression suppresses osteosarcoma cell growth in vitro and in vivo

  • Authors:
    • Weitao Yao
    • Xin Wang
    • Qiqing Cai
    • Songtao Gao
    • Jiaqiang Wang
    • Peng Zhang
  • View Affiliations

  • Published online on: September 29, 2014     https://doi.org/10.3892/ijmm.2014.1948
  • Pages: 1655-1660
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Abstract

Tumor necrosis factor (TNF) receptor‑associated factor 4 (TRAF4) is an adapter molecule that is overexpressed in certain cancers. TRAF4 is overexpressed in osteosarcoma tissues and osteosarcoma cells. Using the technique of RNA interference, the expression of TRAF4 in the human osteosarcoma Saos‑2 cell line was shown to be downregulated. The proliferation, cell cycle arrest and apoptosis ability of Saos‑2 cells were examined, as was tumor development in a xenograft mouse model. The results showed that the TRAF4 knockdown exerts inhibitory effects on the proliferation ability of Saos‑2 cells and tumor development in a xenograft mouse model. Simultaneously, it was found that TRAF4 knockdown led to cell cycle arrest in the G1 phase and promoted Saos‑2 cell apoptosis. Following TNF‑α treatment, the expression of nuclear factor κB was significantly reduced in the TRAF4‑small interfering RNA group. These results indicate that TRAF4 regulated osteosarcoma cell growth in vitro and in vivo, and offers a candidate molecular target for osteosarcoma prevention and therapy.
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December-2014
Volume 34 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yao W, Wang X, Cai Q, Gao S, Wang J and Zhang P: Knockdown of TRAF4 expression suppresses osteosarcoma cell growth in vitro and in vivo. Int J Mol Med 34: 1655-1660, 2014
APA
Yao, W., Wang, X., Cai, Q., Gao, S., Wang, J., & Zhang, P. (2014). Knockdown of TRAF4 expression suppresses osteosarcoma cell growth in vitro and in vivo. International Journal of Molecular Medicine, 34, 1655-1660. https://doi.org/10.3892/ijmm.2014.1948
MLA
Yao, W., Wang, X., Cai, Q., Gao, S., Wang, J., Zhang, P."Knockdown of TRAF4 expression suppresses osteosarcoma cell growth in vitro and in vivo". International Journal of Molecular Medicine 34.6 (2014): 1655-1660.
Chicago
Yao, W., Wang, X., Cai, Q., Gao, S., Wang, J., Zhang, P."Knockdown of TRAF4 expression suppresses osteosarcoma cell growth in vitro and in vivo". International Journal of Molecular Medicine 34, no. 6 (2014): 1655-1660. https://doi.org/10.3892/ijmm.2014.1948