Effects of lactic acid bacteria on cardiac apoptosis are mediated by activation of the phosphatidylinositol-3 kinase/AKT survival-signalling pathway in rats fed a high-fat diet

  • Authors:
    • Hsueh‑Fang Wang
    • Pei‑Pei Lin
    • Chun‑Hua Chen
    • Yu‑Lan Yeh
    • Chun‑Chih Huang
    • Chih‑Yang Huang
    • Cheng‑Chih Tsai
  • View Affiliations

  • Published online on: December 4, 2014     https://doi.org/10.3892/ijmm.2014.2021
  • Pages: 460-470
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Abstract

Through a high-fat diet, obesity leads to cardiomyocyte dysfunction and apoptosis. In addition, there is no evidence that probiotics have potential health effects associated with cardiac apoptosis in obese rats. The present study aimed to explore the effects of probiotics on obesity and cardiac apoptosis in rats fed a high-fat diet (HF). Eight‑week‑old male Wistar rats were separated randomly into five equally sized experimental groups: Normal diet (NC) and high-fat diet (HFC) groups, and high-fat diet supplemented with low (HFL), medium (HFM) or high (HFH) doses of multi‑strain probiotics groups. The rats were subsequently studied for 8 weeks. Food intake and body weights were recorded following sacrifice, and food utilization rates, body fat and serum cholesterol levels were analysed. The myocardial architecture of the left ventricle was evaluated by hematoxylin‑eosin staining, and key apoptotic‑related pathway molecules were analysed by western blotting. Rat weights and triglyceride levels were decreased with oral administration of high doses of probiotics (HFH) compared to the HFC group. Abnormal myocardial architecture and enlarged interstitial spaces were observed in HFC hearts, but were significantly decreased in groups that were provided multi‑strain probiotics compared with NC hearts. Western blot analysis demonstrated that key components of the Fas receptor‑ and mitochondrial‑dependent apoptotic pathways were significantly suppressed in multi‑strain probiotic treated groups compared to the HF group. Additionally, cardiac insulin, such as the insulin‑like growth factor I receptor (IGFIR)‑dependent survival signalling components, were highly induced in left ventricles from rats administered probiotics. Together, these findings strongly suggest that oral administration of probiotics may attenuate cardiomyocyte apoptosis by activation of the phosphatidylinositol‑3 kinase/AKT survival‑signalling pathway in obese rats.
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February-2015
Volume 35 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang HF, Lin PP, Chen CH, Yeh YL, Huang CC, Huang CY and Tsai CC: Effects of lactic acid bacteria on cardiac apoptosis are mediated by activation of the phosphatidylinositol-3 kinase/AKT survival-signalling pathway in rats fed a high-fat diet. Int J Mol Med 35: 460-470, 2015
APA
Wang, H., Lin, P., Chen, C., Yeh, Y., Huang, C., Huang, C., & Tsai, C. (2015). Effects of lactic acid bacteria on cardiac apoptosis are mediated by activation of the phosphatidylinositol-3 kinase/AKT survival-signalling pathway in rats fed a high-fat diet. International Journal of Molecular Medicine, 35, 460-470. https://doi.org/10.3892/ijmm.2014.2021
MLA
Wang, H., Lin, P., Chen, C., Yeh, Y., Huang, C., Huang, C., Tsai, C."Effects of lactic acid bacteria on cardiac apoptosis are mediated by activation of the phosphatidylinositol-3 kinase/AKT survival-signalling pathway in rats fed a high-fat diet". International Journal of Molecular Medicine 35.2 (2015): 460-470.
Chicago
Wang, H., Lin, P., Chen, C., Yeh, Y., Huang, C., Huang, C., Tsai, C."Effects of lactic acid bacteria on cardiac apoptosis are mediated by activation of the phosphatidylinositol-3 kinase/AKT survival-signalling pathway in rats fed a high-fat diet". International Journal of Molecular Medicine 35, no. 2 (2015): 460-470. https://doi.org/10.3892/ijmm.2014.2021