Open Access

An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-α-stimulated human aortic smooth muscle cells through the NF-κB pathway

  • Authors:
    • Hyun Jung Koo
    • Eun-Hwa Sohn
    • Suhkneung Pyo
    • Han Goo Woo
    • Dae Won Park
    • Young-Min Ham
    • Seon-A Jang
    • Soo-Yeong Park
    • Se Chan Kang
  • View Affiliations

  • Published online on: February 26, 2015     https://doi.org/10.3892/ijmm.2015.2112
  • Pages: 915-924
  • Copyright: © Koo et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The root of Cynanchum wilfordii (C. wilfordii) contains several biologically active compounds which have been used as traditional medicines in Asia. In the present study, we evaluated the anti-inflammatory effects of an ethanol root extract of C. wilfordii (CWE) on tumor necrosis factor (TNF)-α-stimulated human aortic smooth muscle cells (HASMCs). The inhibitory effects of CWE on vascular cell adhesion molecule (VCAM)-1 expression under an optimum extraction condition were examined. CWE suppressed the expression of VCAM-1 and ICAM-1 and the adhesion of THP-1 monocytes to the TNF-α-stimulated HASMCs. Consistent with the in vitro observations, CWE inhibited the aortic expression of ICAM-1 and VCAM-1 in atherogenic diet-fed mice. CWE also downregulated the expression of nuclear factor-κB (NF-κB p65) and its uclear translocation in the stimulated HASMCs. In order to identify the active components in CWE, we re-extracted CWE using several solvents, and found that the ethyl acetate fraction was the most effective in suppressing the expression of VCAM-1 and ICAM-1. Four major acetophenones were purified from the ethyl acetate fraction, and two components, p-hydroxyacetophenone and cynandione A, potently inhibited the expression of ICAM-1 and VCAM-1 in the stimulated HASMCs. We assessed and determined the amounts of these two active components from CWE, and our results suggested that the root of C. wilfordii and its two bioactive acetophenones may be used for the prevention and treatment of atherosclerosis and vascular inflammatory diseases.
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April-2015
Volume 35 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Koo HJ, Sohn E, Pyo S, Woo HG, Park DW, Ham Y, Jang S, Park S and Kang SC: An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-α-stimulated human aortic smooth muscle cells through the NF-κB pathway. Int J Mol Med 35: 915-924, 2015
APA
Koo, H.J., Sohn, E., Pyo, S., Woo, H.G., Park, D.W., Ham, Y. ... Kang, S.C. (2015). An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-α-stimulated human aortic smooth muscle cells through the NF-κB pathway. International Journal of Molecular Medicine, 35, 915-924. https://doi.org/10.3892/ijmm.2015.2112
MLA
Koo, H. J., Sohn, E., Pyo, S., Woo, H. G., Park, D. W., Ham, Y., Jang, S., Park, S., Kang, S. C."An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-α-stimulated human aortic smooth muscle cells through the NF-κB pathway". International Journal of Molecular Medicine 35.4 (2015): 915-924.
Chicago
Koo, H. J., Sohn, E., Pyo, S., Woo, H. G., Park, D. W., Ham, Y., Jang, S., Park, S., Kang, S. C."An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-α-stimulated human aortic smooth muscle cells through the NF-κB pathway". International Journal of Molecular Medicine 35, no. 4 (2015): 915-924. https://doi.org/10.3892/ijmm.2015.2112