Cysteine induces longitudinal bone growth in mice by upregulating IGF-I

  • Authors:
    • Phil‑Dong Moon
    • Min‑Ho Kim
    • Hyun‑A Oh
    • Sun‑Young Nam
    • Na‑Ra Han
    • Hyun‑Ja Jeong
    • Hyung‑Min Kim
  • View Affiliations

  • Published online on: June 23, 2015     https://doi.org/10.3892/ijmm.2015.2257
  • Pages: 571-576
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Abstract

Cysteine (Cys) is known to exert various effects, such as antioxidant, antipancreatitic and antidiabetic effects. However, the effects of Cys on longitudinal bone growth have not been elucidate to date. Thus, the aim of the present study was to evaluate the effects of Cys on bone growth. Growth‑plate thickness and bone parameters, such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connectivity density (Conn.D) and total porosity were analyzed by means of micro-computed tomography (µCT). The levels of serum insulin‑like growth factor‑I (IGF‑I) were measured by enzyme‑linked immunosorbent assay (ELISA). Hepatic IGF‑I mRNA expression was analyzed by quantitative polymerase chain reaction (qPCR). The phosphorylation of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) was investigated by western blot analysis. Our results revealed that Cys increased IGF‑I mRNA expression in HepG2 cells. The thickness of the growth plates was increased following treatment with Cys. Moreover, BV/TV, Tb.Th, TbN, Conn.D and total porosity were improved following treatment with Cys. Hepatic IGF‑I mRNA expression and serum IGF‑I levels were increased by Cys. The levels of phosphorylated JAK2 and STAT5 were elevated by Cys. The findings of our study indicate that Cys increases the thickness of growth plates through the upregulation of IGF‑I, which results from the phosphorylation of JAK2-STAT5. Thus, our data suggest that Cys may have potential for use as a growth-promoting agent.
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August-2015
Volume 36 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Moon PD, Kim MH, Oh HA, Nam SY, Han NR, Jeong HJ and Kim HM: Cysteine induces longitudinal bone growth in mice by upregulating IGF-I. Int J Mol Med 36: 571-576, 2015
APA
Moon, P., Kim, M., Oh, H., Nam, S., Han, N., Jeong, H., & Kim, H. (2015). Cysteine induces longitudinal bone growth in mice by upregulating IGF-I. International Journal of Molecular Medicine, 36, 571-576. https://doi.org/10.3892/ijmm.2015.2257
MLA
Moon, P., Kim, M., Oh, H., Nam, S., Han, N., Jeong, H., Kim, H."Cysteine induces longitudinal bone growth in mice by upregulating IGF-I". International Journal of Molecular Medicine 36.2 (2015): 571-576.
Chicago
Moon, P., Kim, M., Oh, H., Nam, S., Han, N., Jeong, H., Kim, H."Cysteine induces longitudinal bone growth in mice by upregulating IGF-I". International Journal of Molecular Medicine 36, no. 2 (2015): 571-576. https://doi.org/10.3892/ijmm.2015.2257