Growth-inhibitory and chemosensitizing effects of microRNA-31 in human glioblastoma multiforme cells

  • Authors:
    • Rong-Jing Zhou
    • Xiong-Ying Xu
    • Bu-Xing Liu
    • Wen-Zhen Dai
    • Mei-Qin Cai
    • Chun-Feng Bai
    • Xian-Fei Zhang
    • Li-Min Wang
    • Li Lin
    • Shu-Zhen Jia
    • Wen-Hua Wang
  • View Affiliations

  • Published online on: August 13, 2015     https://doi.org/10.3892/ijmm.2015.2312
  • Pages: 1159-1164
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Abstract

The constitutive activation of signal transducer and activator of transcription 3 (STAT3) contributes to resistance to temozolomide (TMZ) in glioblastoma multiforme (GBM). The aim of this study was to explore the biological role of microRNA-31 (miR-31) in GBM, particularly its role in the regulation of TMZ chemosensitivity. For this purpose, the human GBM cell lines, U251 and U87, were transfected with a miR-31 precursor (pre-miR-31), and cell proliferation, apoptosis and STAT3 phosphorylation were then assessed. To evaluate the effects of miR-31 on TMZ cytotoxicity, the cells were transfected with pre-miR-31 and exposed to 100 µM TMZ for 72 h prior to cell proliferation and apoptosis analysis. A constitutively active STAT3 mutant was co-transfected with pre-miR-31 into the cells to confirm the mediating role of STAT3 signaling. The enforced expression of miR-31 significantly reduced cell proliferation and induced mitochondrial apoptosis, as manifested by the loss of mitochondrial membrane potential and the increase in caspase-9 and caspase-3 activity. The phosphorylation level of STAT3 was significantly decreased by the overexpression of miR-31. The co-delivery of the constitutively active STAT3 mutant blocked the tumor suppressive effects of miR-31. In addition, miR-31 overexpression significantly enhanced the cytotoxic effects of TMZ on the GBM cells, as evidenced by the accelerated suppression of cell proliferation and the induction of apoptosis. The chemosensitizing effects of miR-31 were significantly impaired by the expression of the constitutively active STAT3 mutant. Taken together, our results indicate that miR-31 triggers mitochondrial apoptosis and potentiates TMZ cytotoxicity in GBM cells largely through the suppression of STAT3 activation. Thus, the restoration of miR-31 expression may be of therapeutic beenefit in the treatment of GBM.
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October-2015
Volume 36 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhou R, Xu X, Liu B, Dai W, Cai M, Bai C, Zhang X, Wang L, Lin L, Jia S, Jia S, et al: Growth-inhibitory and chemosensitizing effects of microRNA-31 in human glioblastoma multiforme cells. Int J Mol Med 36: 1159-1164, 2015
APA
Zhou, R., Xu, X., Liu, B., Dai, W., Cai, M., Bai, C. ... Wang, W. (2015). Growth-inhibitory and chemosensitizing effects of microRNA-31 in human glioblastoma multiforme cells. International Journal of Molecular Medicine, 36, 1159-1164. https://doi.org/10.3892/ijmm.2015.2312
MLA
Zhou, R., Xu, X., Liu, B., Dai, W., Cai, M., Bai, C., Zhang, X., Wang, L., Lin, L., Jia, S., Wang, W."Growth-inhibitory and chemosensitizing effects of microRNA-31 in human glioblastoma multiforme cells". International Journal of Molecular Medicine 36.4 (2015): 1159-1164.
Chicago
Zhou, R., Xu, X., Liu, B., Dai, W., Cai, M., Bai, C., Zhang, X., Wang, L., Lin, L., Jia, S., Wang, W."Growth-inhibitory and chemosensitizing effects of microRNA-31 in human glioblastoma multiforme cells". International Journal of Molecular Medicine 36, no. 4 (2015): 1159-1164. https://doi.org/10.3892/ijmm.2015.2312