EGCG attenuates atherosclerosis through the Jagged-1/Notch pathway

  • Authors:
    • Jianguo Yin
    • Fang Huang
    • Yuhong Yi
    • Liang Yin
    • Daoquan Peng
  • View Affiliations

  • Published online on: December 1, 2015     https://doi.org/10.3892/ijmm.2015.2422
  • Pages: 398-406
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Abstract

Atherosclerosis is the most common cause of cardiovascular diseases worldwide. Oxidized low-density lipoprotein (ox-LDL) is a particularly important risk factor in the pathogenesis of atherosclerosis. Accumulating evidence has indicated that epigallocatechin-3-gallate (EGCG; a catechin found in the popular beverage, greent tea) protects against ox-LDL-induced atherosclerosis. However, the underlying mechanisms remain unclear. In the present study, ox-LDL (100 mg/l) induced damage to, and the apoptosis of human umbilical vein endothelial cells (HUVECs) by reducing endothelial nitric oxide synthase (eNOS) expression and promoting inducible nitric oxide synthase (iNOS) expression; these effects were abrogated by the addition of 50 µM EGCG. Furthermore, ox-LDL rapidly activated the membrane translocation of p22phox, and altered the protein expression of Jagged-1 and Notch pathway-related proteins [Math1, hairy and enhancer of split (HES)1 and HES5]; these effects were also prevented by pre-treatment with 50 µM EGCG. In addition, Jagged-1 played a significant role in the EGCG-mediated protection against ox-LDL-induced apoptosis and ox-LDL‑diminished cell adhesion in the HUVECs. Finally, EGCG inhibited high-fat diet (HFD)-induced atherosclerosis in apolipoprotein E (ApoE) knockout (ApoE-KO) mice through the Jagged-1/Notch pathway. Taken together, these findings demonstrate that 50 µM EGCG protects against ox-LDL-induced endothelial dysfunction through the Jagged-1/Notch signaling pathway. Moreover, our data provide insight into the possible molecular mechanisms through which EGCG attenuates ox-LDL‑induced vascular endothelial dysfunction.
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February-2016
Volume 37 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yin J, Huang F, Yi Y, Yin L and Peng D: EGCG attenuates atherosclerosis through the Jagged-1/Notch pathway. Int J Mol Med 37: 398-406, 2016
APA
Yin, J., Huang, F., Yi, Y., Yin, L., & Peng, D. (2016). EGCG attenuates atherosclerosis through the Jagged-1/Notch pathway. International Journal of Molecular Medicine, 37, 398-406. https://doi.org/10.3892/ijmm.2015.2422
MLA
Yin, J., Huang, F., Yi, Y., Yin, L., Peng, D."EGCG attenuates atherosclerosis through the Jagged-1/Notch pathway". International Journal of Molecular Medicine 37.2 (2016): 398-406.
Chicago
Yin, J., Huang, F., Yi, Y., Yin, L., Peng, D."EGCG attenuates atherosclerosis through the Jagged-1/Notch pathway". International Journal of Molecular Medicine 37, no. 2 (2016): 398-406. https://doi.org/10.3892/ijmm.2015.2422