Influence of different forms of fluid shear stress on vascular endothelial TGF-beta1 mRNA expression.

  • Authors:
    • R M Lum
    • L M Wiley
    • A I Barakat
  • View Affiliations

  • Published online on: June 1, 2000     https://doi.org/10.3892/ijmm.5.6.635
  • Pages: 635-676
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Abstract

This study investigated the effects of long-term exposure of steady (19 dyne/cm2), 1-Hz non-reversing pulsatile (19+/-6 dyne/cm2) and 1-Hz purely oscillatory (0+/-19 dyne/cm2) shear stress on endothelial transforming growth factor-beta1 (TGF-beta1) mRNA expression. Cultured bovine aortic endothelial cells (BAECs) were systematically exposed to the three flow conditions for periods of 2, 6, 12 and 24 h, and relative differences in TGF-beta1 mRNA levels were measured by semi-quantitative RT-PCR. In response to steady shear stress, TGF-beta1 mRNA levels normalized to no-flow controls were 1.24, 1.42, 1.30 and 1.47 at the 2-, 6-, 12- and 24-h time points, respectively. In response to non-reversing pulsatile flow, these levels were 1.49, 1.64, 1.64 and 1.73, while the respective transcript levels for oscillatory flow were 1.33, 1.12, 1.12 and 1. 93. These results indicate that BAEC TGF-beta1 mRNA was up-regulated with the kinetics of the up-regulation faster for steady and non-reversing pulsatile flow than for oscillatory flow. Given the preferential localization of early atherosclerotic lesions in arterial regions exposed to low and/or oscillatory shear stress and the implication of TGF-beta1 as an athero-protective gene, these results are consistent with the notion that regions transiently exposed to oscillatory flow may be particularly prone to atherosclerosis.

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Jun 2000
Volume 5 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lum R, Wiley L and Barakat A: Influence of different forms of fluid shear stress on vascular endothelial TGF-beta1 mRNA expression.. Int J Mol Med 5: 635-676, 2000
APA
Lum, R., Wiley, L., & Barakat, A. (2000). Influence of different forms of fluid shear stress on vascular endothelial TGF-beta1 mRNA expression.. International Journal of Molecular Medicine, 5, 635-676. https://doi.org/10.3892/ijmm.5.6.635
MLA
Lum, R., Wiley, L., Barakat, A."Influence of different forms of fluid shear stress on vascular endothelial TGF-beta1 mRNA expression.". International Journal of Molecular Medicine 5.6 (2000): 635-676.
Chicago
Lum, R., Wiley, L., Barakat, A."Influence of different forms of fluid shear stress on vascular endothelial TGF-beta1 mRNA expression.". International Journal of Molecular Medicine 5, no. 6 (2000): 635-676. https://doi.org/10.3892/ijmm.5.6.635