Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes.

  • Authors:
    • T Takaki
    • A Hiraki
    • A Uenaka
    • S Gomi
    • K Itoh
    • H Udono
    • A Shibuya
    • T Tsuji
    • S Sekiguchi
    • E Nakayama
  • View Affiliations

  • Published online on: May 1, 1998     https://doi.org/10.3892/ijo.12.5.1103
  • Pages: 1103-1112
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Abstract

Cytotoxic T lymphocytes (CTL) specific for HLA-A2-binding MAGE-3 peptide (FLWGPRALV) were generated by repetitive stimulation of PBMC with the peptide in the presence of EBV-transformed B blasts and IL-2. Using these CTL, we investigated the expression of the HLA-A2-binding MAGE-3 peptide on lung cancer cell lines. Of 14 cell lines investigated, 1-87, PC-9, OU-LC-KI, 11-18 and LK87 were derived from HLA-A2 positive patients. But cytofluorometry analysis showed that 1-87, PC-9 and OU-LC-KI, but not 11-18 or LK87 expressed the HLA-A2 antigen. All five cell lines expressed MAGE-3 gene mRNA. Twelve of thirteen CTL lines from two HLA-A2 positive donors showed no cytotoxicity against any of the 14 lung cancer cell lines. CTL line TI-1 showed cytotoxicity against 1-87 but not against any of the other cell lines. Treatment of 1-87 with IFN-gamma greatly augmented the cytotoxicity of TI-1 and induced it in the other 12 CTL lines, confirming the expression of the peptide on 1-87. No cytotoxicity was induced by IFN-gamma treatment of PC-9 or OU-LC-KI. However, PC-9 and OU-LC-KI pulsed with the peptide were killed efficiently by all of the CTL lines, suggesting no expression of the peptide on those cells. A low level of cytotoxicity was induced on 11-18 but not LK87 by IFN-gamma treatment, although expression of the HLA-A2 antigen was not observed by cytofluorometry. These findings showed that expression of the HLA-A2-binding MAGE-3 peptide recognized by CTL was variable on lung cancer cell lines.

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May 1998
Volume 12 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Takaki T, Hiraki A, Uenaka A, Gomi S, Itoh K, Udono H, Shibuya A, Tsuji T, Sekiguchi S, Nakayama E, Nakayama E, et al: Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes.. Int J Oncol 12: 1103-1112, 1998
APA
Takaki, T., Hiraki, A., Uenaka, A., Gomi, S., Itoh, K., Udono, H. ... Nakayama, E. (1998). Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes.. International Journal of Oncology, 12, 1103-1112. https://doi.org/10.3892/ijo.12.5.1103
MLA
Takaki, T., Hiraki, A., Uenaka, A., Gomi, S., Itoh, K., Udono, H., Shibuya, A., Tsuji, T., Sekiguchi, S., Nakayama, E."Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes.". International Journal of Oncology 12.5 (1998): 1103-1112.
Chicago
Takaki, T., Hiraki, A., Uenaka, A., Gomi, S., Itoh, K., Udono, H., Shibuya, A., Tsuji, T., Sekiguchi, S., Nakayama, E."Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes.". International Journal of Oncology 12, no. 5 (1998): 1103-1112. https://doi.org/10.3892/ijo.12.5.1103