Analysis of the candidate target genes for mutation in microsatellite instability-positive cancers of the colorectum, stomach, and endometrium.

  • Authors:
    • S Semba
    • H Ouyang
    • S Y Han
    • Y Kato
    • A Horii
  • View Affiliations

  • Published online on: Saturday, April 1, 2000
  • Pages: 731-738
  • DOI: 10.3892/ijo.16.4.731

Abstract

Microsatellite instability (MSI) in human carcinoma DNA is a characteristic phenotype observed in hereditary non-polyposis colorectal cancer and also in some human sporadic cancers including multiple primary carcinomas. In this study, we analyzed mutations in the hCHK1, E2F4, hMSH3, and hMSH6 genes in MSI+ human cancers arising in colorectum, stomach and endometrium. The E2F4 and hMSH3 genes were mutated in all tumor types. Interestingly, the hMSH6 gene was mutated in colorectal and gastric cancers but not in endometrial cancer; this is similar to the TGFbetaRII gene. It is notable that the mutation status of the secondary mutators, hMSH3 and hMSH6, did not influence slippage-related frameshift mutations in genes harboring simple tandem-repeats, which suggests that the MSI phenotype may be affected mainly by abnormalities in the primary mutator genes, not by the secondary mutator genes. No mutations were observed in the cell cycle checkpoint gene hCHK1; mutations of this gene are thought to have a limited role, if any, in at least the tumor types analyzed in this study.
Journal Cover

April 2000
Volume 16 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Semba, S., Ouyang, H., Han, S., Kato, Y., & Horii, A. (2000). Analysis of the candidate target genes for mutation in microsatellite instability-positive cancers of the colorectum, stomach, and endometrium.. International Journal of Oncology, 16(4), 731-738.
MLA
Semba, Ouyang, Han, Kato, and A Horii. "Analysis of the candidate target genes for mutation in microsatellite instability-positive cancers of the colorectum, stomach, and endometrium.." International Journal of Oncology International Journal of Oncology 16.4 (2000): 731-738.
Chicago
Semba, Ouyang, Han, Kato, and A Horii. "Analysis of the candidate target genes for mutation in microsatellite instability-positive cancers of the colorectum, stomach, and endometrium.." International Journal of Oncology International Journal of Oncology 16 no. 4 (2000): 731-738.