A novel role for the urokinase-type plasminogen activator receptor in apoptosis of malignant gliomas.

  • Authors:
    • Y Kin
    • S K Chintala
    • Y Go
    • R Sawaya
    • S Mohanam
    • A P Kyritsis
    • J S Rao
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/ijo.17.1.61
  • Pages: 61-66
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Abstract

Glioblastomas express more urokinase-type plasminogen activator receptor (uPAR) than do low-grade gliomas and normal brain tissue. We previously showed that downregulation of uPAR through the transfection of SNB19 cells with an antisense cDNA construct corresponding to 300 bp of the 5' end of the human uPAR gene inhibited tumor cell invasion in vitro and tumor formation in vivo. Here we sought to determine whether uPAR is necessary for cell survival and whether the inhibition of tumor formation in nude mice is due to apoptosis of intracerebrally injected SNB19 cells. Apoptosis measured by DNA fragmentation were higher in the brains of animals injected with the antisense stable transfectants than in those injected with the parental cells. Moreover, the increase in apoptotic cell death in vitro was associated with increased expression of apoptotic protein BAX in antisense clones compared to controls. To our knowledge, this is the first report of uPAR playing a novel role in cell survival in human gliomas.

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Jul 2000
Volume 17 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kin Y, Chintala S, Go Y, Sawaya R, Mohanam S, Kyritsis A and Rao J: A novel role for the urokinase-type plasminogen activator receptor in apoptosis of malignant gliomas.. Int J Oncol 17: 61-66, 2000
APA
Kin, Y., Chintala, S., Go, Y., Sawaya, R., Mohanam, S., Kyritsis, A., & Rao, J. (2000). A novel role for the urokinase-type plasminogen activator receptor in apoptosis of malignant gliomas.. International Journal of Oncology, 17, 61-66. https://doi.org/10.3892/ijo.17.1.61
MLA
Kin, Y., Chintala, S., Go, Y., Sawaya, R., Mohanam, S., Kyritsis, A., Rao, J."A novel role for the urokinase-type plasminogen activator receptor in apoptosis of malignant gliomas.". International Journal of Oncology 17.1 (2000): 61-66.
Chicago
Kin, Y., Chintala, S., Go, Y., Sawaya, R., Mohanam, S., Kyritsis, A., Rao, J."A novel role for the urokinase-type plasminogen activator receptor in apoptosis of malignant gliomas.". International Journal of Oncology 17, no. 1 (2000): 61-66. https://doi.org/10.3892/ijo.17.1.61