Expression and function of interleukin-8 in human hepatocellular carcinoma

  • Authors:
    • Jun Akiba
    • Hirohisa Yano
    • Sachiko Ogasawara
    • Koichi Higaki
    • M. Kojiro
  • View Affiliations

  • Published online on: February 1, 2001     https://doi.org/10.3892/ijo.18.2.257
  • Pages: 257-264
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Abstract

Expression and functions of interleukin (IL)-8, a pro-inflammatory cytokine with angiogenesis action, was examined in 23 surgically resected hepatocellular carcinoma (HCC) specimens and 7 HCC cell lines. In all HCC tissues, IL-8 expression was confirmed with reverse-transcription polymerase chain reaction method and enzyme-linked immunosorbent assay, and immunohistochemistry showed HCC cells were the major producer of IL-8 in the tissues. Microvessel density was measured by the double immunohistochemical staining of muscular vessels in HCC tissues, but the density was not related to the level of IL-8 in the HCC tissues. On the other hand, in the co-culture of human umbilical vein endothelial cells (HUVEC) and a HCC cell line (KIM-1), IL-8 produced by KIM-1 significantly accelerated the proliferation of HUVEC. In addition, cases with a high IL-8 level in cancerous tissue had a significantly higher frequency of portal vein invasion, venous invasion and bile duct invasion (p<0.05). In the cultures of 7 HCC cell lines IL-8 secretion into culture medium increased with the treatment of IL-1β or tumor necrosis factor-α. This showed IL-8 expression is regulated by inflammatory cytokines. IL-8 produced by HCC is an angiogenesis factor of HCC, but it could have a much more important role in the invasion and metastasis of HCC.

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February 2001
Volume 18 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Akiba J, Yano H, Ogasawara S, Higaki K and Kojiro M: Expression and function of interleukin-8 in human hepatocellular carcinoma. Int J Oncol 18: 257-264, 2001
APA
Akiba, J., Yano, H., Ogasawara, S., Higaki, K., & Kojiro, M. (2001). Expression and function of interleukin-8 in human hepatocellular carcinoma. International Journal of Oncology, 18, 257-264. https://doi.org/10.3892/ijo.18.2.257
MLA
Akiba, J., Yano, H., Ogasawara, S., Higaki, K., Kojiro, M."Expression and function of interleukin-8 in human hepatocellular carcinoma". International Journal of Oncology 18.2 (2001): 257-264.
Chicago
Akiba, J., Yano, H., Ogasawara, S., Higaki, K., Kojiro, M."Expression and function of interleukin-8 in human hepatocellular carcinoma". International Journal of Oncology 18, no. 2 (2001): 257-264. https://doi.org/10.3892/ijo.18.2.257