Role of galectin-3 in adenocarcinoma liver metastasis

  • Authors:
    • Haruhiko Inufusa
    • Masato Nakamura
    • Toshiyuki Adachi
    • Miho Aga
    • Masashi Kurimoto
    • Yoshihiro Nakatani
    • Tsukasa Wakano
    • Masayuki Miyake
    • Kiyotaka Okuno
    • Hitoshi Shiozaki
    • Masayuki Yasutomi
  • View Affiliations

  • Published online on: November 1, 2001     https://doi.org/10.3892/ijo.19.5.913
  • Pages: 913-919
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Abstract

Galectin-3 is a lactosamine-specific lectin that binds to laminin sugar-sites, and up-regulated expression of galectin-3 in primary colorectal cancer is involved in cancer progression and metastasis. Inhibitory effects of cell adhesion and liver metastasis of adenocarcinoma via portal vein by lectin-binding sugar and anti-galectin-3 antibody was examined to determine the role of galectin-laminin binding in cancer liver metastasis. Highly metastatic adenocarcinoma cell lines XK4-A3 and RPMI4788 were used in in vitro cell attachment and nude mice liver metastatic experiments, and inhibitory effects of anti-galectin-3 antibody or lectin-binding sugars were examined. The in vitro adhesion assay demonstrated that the anti-galectin-3 antibody and α-lactose inhibited XK4-A3 and RPMI4788 cell adhesion to laminin in a dose-dependent manner. The liver metastasis of XK4-A3 and RPMI4788 was reduced 50 and 60%, respectively (P<0.001) by α-lactose treatment. Anti-galectin-3 antibody also inhibited liver metastasis in a dose-dependent manner, and maximum inhibition rate was 66% for XK4-A3 and 90% for RPMI4788. Galectin-3 plays an important role in liver metastasis of adenocarcinoma by the mechanisms of galectin-3 binding to laminin. Inhibition of galectin-3 on cancer cell surface induces reduced cell attachment to laminin and liver metastasis.

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November 2001
Volume 19 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Inufusa H, Nakamura M, Adachi T, Aga M, Kurimoto M, Nakatani Y, Wakano T, Miyake M, Okuno K, Shiozaki H, Shiozaki H, et al: Role of galectin-3 in adenocarcinoma liver metastasis. Int J Oncol 19: 913-919, 2001
APA
Inufusa, H., Nakamura, M., Adachi, T., Aga, M., Kurimoto, M., Nakatani, Y. ... Yasutomi, M. (2001). Role of galectin-3 in adenocarcinoma liver metastasis. International Journal of Oncology, 19, 913-919. https://doi.org/10.3892/ijo.19.5.913
MLA
Inufusa, H., Nakamura, M., Adachi, T., Aga, M., Kurimoto, M., Nakatani, Y., Wakano, T., Miyake, M., Okuno, K., Shiozaki, H., Yasutomi, M."Role of galectin-3 in adenocarcinoma liver metastasis". International Journal of Oncology 19.5 (2001): 913-919.
Chicago
Inufusa, H., Nakamura, M., Adachi, T., Aga, M., Kurimoto, M., Nakatani, Y., Wakano, T., Miyake, M., Okuno, K., Shiozaki, H., Yasutomi, M."Role of galectin-3 in adenocarcinoma liver metastasis". International Journal of Oncology 19, no. 5 (2001): 913-919. https://doi.org/10.3892/ijo.19.5.913