Role of galectin-3 in adenocarcinoma liver metastasis

  • Authors: Haruhiko Inufusa, Masato Nakamura, Toshiyuki Adachi, Miho Aga, Masashi Kurimoto, Yoshihiro Nakatani, Tsukasa Wakano, Masayuki Miyake, Kiyotaka Okuno, Hitoshi Shiozaki, Masayuki Yasutomi
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  • Published online on: Thursday, November 1, 2001
  • Pages: 913-919
  • DOI: 10.3892/ijo.19.5.913

Abstract

Galectin-3 is a lactosamine-specific lectin that binds to laminin sugar-sites, and up-regulated expression of galectin-3 in primary colorectal cancer is involved in cancer progression and metastasis. Inhibitory effects of cell adhesion and liver metastasis of adenocarcinoma via portal vein by lectin-binding sugar and anti-galectin-3 antibody was examined to determine the role of galectin-laminin binding in cancer liver metastasis. Highly metastatic adenocarcinoma cell lines XK4-A3 and RPMI4788 were used in in vitro cell attachment and nude mice liver metastatic experiments, and inhibitory effects of anti-galectin-3 antibody or lectin-binding sugars were examined. The in vitro adhesion assay demonstrated that the anti-galectin-3 antibody and α-lactose inhibited XK4-A3 and RPMI4788 cell adhesion to laminin in a dose-dependent manner. The liver metastasis of XK4-A3 and RPMI4788 was reduced 50 and 60%, respectively (P<0.001) by α-lactose treatment. Anti-galectin-3 antibody also inhibited liver metastasis in a dose-dependent manner, and maximum inhibition rate was 66% for XK4-A3 and 90% for RPMI4788. Galectin-3 plays an important role in liver metastasis of adenocarcinoma by the mechanisms of galectin-3 binding to laminin. Inhibition of galectin-3 on cancer cell surface induces reduced cell attachment to laminin and liver metastasis.
Journal Cover

November 2001
Volume 19 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Inufusa, H., Nakamura, M., Adachi, T., Aga, M., Kurimoto, M., Nakatani, Y., Wakano, T., Miyake, M., Okuno, K., Shiozaki, H., & Yasutomi, M. (2001). Role of galectin-3 in adenocarcinoma liver metastasis. International Journal of Oncology, 19(5), 913-919.
MLA
Inufusa, Nakamura, Adachi, Aga, Kurimoto, Nakatani, Wakano, Miyake, Okuno, Shiozaki, and Masayuki Yasutomi. "Role of galectin-3 in adenocarcinoma liver metastasis." International Journal of Oncology International Journal of Oncology 19.5 (2001): 913-919.
Chicago
Inufusa, Nakamura, Adachi, Aga, Kurimoto, Nakatani, Wakano, Miyake, Okuno, Shiozaki, and Masayuki Yasutomi. "Role of galectin-3 in adenocarcinoma liver metastasis." International Journal of Oncology International Journal of Oncology 19 no. 5 (2001): 913-919.