Arsenic trioxide induces apoptosis through a reactive oxygen species-dependent pathway and loss of mitochondrial membrane potential in HeLa cells

  • Authors:
    • Sang Hyeok Woo
    • In-Chul Park
    • Myung-Jin Park
    • Hyung-Chahn Lee
    • Su-Jae Lee
    • Yong-Jin Chun
    • Seung-Hoon Lee
    • Seok-Il Hong
    • Chang Hun Rhee
  • View Affiliations

  • Published online on: July 1, 2002     https://doi.org/10.3892/ijo.21.1.57
  • Pages: 57-63
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Abstract

Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage of As2O3 in cervical cancer and its possible mechanisms, human cervical cancer cell line HeLa was employed. The cells underwent apoptosis in response to As2O3, accompanied by a decrease of mitochondrial membrane potential and caspase-3 activation. Overexpression of Bcl-2, however, prevented the dissipation of mitochondrial membrane potential, subsequently protecting the cells from As2O3-induced apoptosis. As2O3 increased cellular content of reactive oxygen species (ROS), especially hydrogen peroxide (H2O2), and the antioxidant N-acetyl-L-cysteine completely suppressed As2O3-induced apoptosis. Furthermore, incubation of the cells with catalase resulted in significant suppression of As2O3-induced apoptosis. The above results indicate that the induction of HeLa cell apoptosis by As2O3 involved an early decrease in cellular mitochondrial membrane potential and increase in ROS content, predominantly H2O2, followed by caspase-3 activation and DNA fragmentation.

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July 2002
Volume 21 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Woo SH, Park I, Park M, Lee H, Lee S, Chun Y, Lee S, Hong S and Rhee CH: Arsenic trioxide induces apoptosis through a reactive oxygen species-dependent pathway and loss of mitochondrial membrane potential in HeLa cells. Int J Oncol 21: 57-63, 2002
APA
Woo, S.H., Park, I., Park, M., Lee, H., Lee, S., Chun, Y. ... Rhee, C.H. (2002). Arsenic trioxide induces apoptosis through a reactive oxygen species-dependent pathway and loss of mitochondrial membrane potential in HeLa cells. International Journal of Oncology, 21, 57-63. https://doi.org/10.3892/ijo.21.1.57
MLA
Woo, S. H., Park, I., Park, M., Lee, H., Lee, S., Chun, Y., Lee, S., Hong, S., Rhee, C. H."Arsenic trioxide induces apoptosis through a reactive oxygen species-dependent pathway and loss of mitochondrial membrane potential in HeLa cells". International Journal of Oncology 21.1 (2002): 57-63.
Chicago
Woo, S. H., Park, I., Park, M., Lee, H., Lee, S., Chun, Y., Lee, S., Hong, S., Rhee, C. H."Arsenic trioxide induces apoptosis through a reactive oxygen species-dependent pathway and loss of mitochondrial membrane potential in HeLa cells". International Journal of Oncology 21, no. 1 (2002): 57-63. https://doi.org/10.3892/ijo.21.1.57