Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines

  • Authors:
    • Shuji Tohda
    • Mai Murata-Ohsawa
    • Seiji Sakano
    • Nobuo Nara
  • View Affiliations

  • Published online on: May 1, 2003     https://doi.org/10.3892/ijo.22.5.1073
  • Pages: 1073-1079
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Abstract

The self-renewal and differentiation of hematopoietic progenitors are regulated by the interaction between Notch receptors and Notch ligands. Since AML originates from dysregulated hematopoietic progenitors, some abnormalities in the Notch system may be involved in the abnormal proliferation of AML cells. However, the significance of the Notch system in AML is not known. We examined the functional roles of Notch activation on the in vitro growth of seven human AML cell lines using three kinds of recombinant Notch ligand proteins, Jagged-1, Delta-1 and Delta-4. The ligands significantly affected the growth of two cell lines. In TMD7 cells, Delta proteins promoted the short-term growth, however, suppressing the self-renewal capacity and long-term growth. In OCI/AML-6 cells, Delta proteins suppressed the growth and self-renewal capacity while inducing differentiation into macrophage-like cells. We additionally found that Notch ligands needed to be immobilized on culture wells to affect the cells. These findings were in contrast to our hypothesis that Notch activation in AML cells leads to excessive self-renewal capacity and proliferation. If the Notch system in AML cells is precisely understood, the control of Notch activation will become a novel therapeutic approach for AML.

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May 2003
Volume 22 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tohda S, Murata-Ohsawa M, Sakano S and Nara N: Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. Int J Oncol 22: 1073-1079, 2003
APA
Tohda, S., Murata-Ohsawa, M., Sakano, S., & Nara, N. (2003). Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines. International Journal of Oncology, 22, 1073-1079. https://doi.org/10.3892/ijo.22.5.1073
MLA
Tohda, S., Murata-Ohsawa, M., Sakano, S., Nara, N."Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines". International Journal of Oncology 22.5 (2003): 1073-1079.
Chicago
Tohda, S., Murata-Ohsawa, M., Sakano, S., Nara, N."Notch ligands, Delta-1 and Delta-4 suppress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines". International Journal of Oncology 22, no. 5 (2003): 1073-1079. https://doi.org/10.3892/ijo.22.5.1073