NAMI-A inhibits the PMA-induced ODC gene expression in ECV304 cells: Involvement of PKC/Raf/Mek/ERK signalling pathway

  • Authors:
    • Marcella Debidda
    • Bastiano Sanna
    • Annalisa Cossu
    • Anna M. Posadino
    • B. Tadolini
    • C. Ventura
    • G. Pintus
  • View Affiliations

  • Published online on: Friday, August 1, 2003
  • Pages: 477-482
  • DOI: 10.3892/ijo.23.2.477

Abstract

Imidazolium trans-imidazole dimethyl sulfoxide tetrachlororuthenate (NAMI-A) is a new compound active against lung metastasis of solid metastasizing tumours. While its in vivo effect has been studied, the molecular insights that underlie its action are largely unknown. Among the possible pathways responsible for malignant transformation, PKC arose as one of the most promising targets for new antineoplastic drugs. We demonstrated the capability of NAMI-A of inhibiting PMA induced-PKC activity in ECV304 in a dose-dependent fashion. Furthermore, NAMI-A through modulation of PKC activity has been proved capable of reducing the phorbol ester induced expression of ornithine decarboxilase (ODC) gene and to abrogate the activation of the Raf/MEK/ERK pathway. Taken together these results suggest that many of the in vivo outcomes of NAMI-A treatment may be the result of a direct action on PKC.
Journal Cover

August 2003
Volume 23 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Debidda, M., Sanna, B., Cossu, A., Posadino, A., Tadolini, B., Ventura, C., & Pintus, G. (2003). NAMI-A inhibits the PMA-induced ODC gene expression in ECV304 cells: Involvement of PKC/Raf/Mek/ERK signalling pathway. International Journal of Oncology, 23(2), 477-482.
MLA
Debidda, Sanna, Cossu, Posadino, Tadolini, Ventura, and G. Pintus. "NAMI-A inhibits the PMA-induced ODC gene expression in ECV304 cells: Involvement of PKC/Raf/Mek/ERK signalling pathway." International Journal of Oncology International Journal of Oncology 23.2 (2003): 477-482.
Chicago
Debidda, Sanna, Cossu, Posadino, Tadolini, Ventura, and G. Pintus. "NAMI-A inhibits the PMA-induced ODC gene expression in ECV304 cells: Involvement of PKC/Raf/Mek/ERK signalling pathway." International Journal of Oncology International Journal of Oncology 23 no. 2 (2003): 477-482.