Targeted inhibition of NMYC by peptide nucleic acid in N-myc amplified human neuroblastoma cells: cell-cycle inhibition with induction of neuronal cell differentiation and apoptosis

  • Authors: Andrea Pession, Roberto Tonelli, Raffaele Fronza, Elena Sciamanna, Roberto Corradini, Stefano Sforza, Tullia Tedeschi, Rosangela Marchelli, Lorenzo Montanaro, Consuelo Camerin, Monica Franzoni, Guido Paolucci
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  • Published online on: Sunday, February 1, 2004
  • Pages: 265-272
  • DOI: 10.3892/ijo.24.2.265

Abstract

We developed an antisense peptide nucleic acid (PNA) targeted against a unique sequence in the terminus of the 5'-UTR of N-myc, designed for selective inhibition of NMYC in neuroblastoma cells. Fluorescent microscopy showed carrier-free delivery of the PNA to two human neuro-blastoma cell lines: GI-LI-N (N-myc-amplified) and GI-CA-N (N-myc-unamplified). Only in the former, PNA treatment determined 70% cell-viability reduction (at 48 h). In N-myc-amplified GI-LI-N cells, the PNA determined NMYC-translation inhibition (Western blotting), accumulation of cells in G1, induction of differentiation and apoptosis. Selectivity of the PNA was demonstrated by altering three point mutations. These findings should encourage development of a PNA-based tumor-specific agent for neuroblastoma (or other neoplasms) with N-myc overexpression.
Journal Cover

February 2004
Volume 24 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Pession, A., Tonelli, R., Fronza, R., Sciamanna, E., Corradini, R., Sforza, S., Tedeschi, T., Marchelli, R., Montanaro, L., Camerin, C., Franzoni, M., & Paolucci, G. (2004). Targeted inhibition of NMYC by peptide nucleic acid in N-myc amplified human neuroblastoma cells: cell-cycle inhibition with induction of neuronal cell differentiation and apoptosis. International Journal of Oncology, 24(2), 265-272.
MLA
Pession, Tonelli, Fronza, Sciamanna, Corradini, Sforza, Tedeschi, Marchelli, Montanaro, Camerin, Franzoni, and Guido Paolucci. "Targeted inhibition of NMYC by peptide nucleic acid in N-myc amplified human neuroblastoma cells: cell-cycle inhibition with induction of neuronal cell differentiation and apoptosis." International Journal of Oncology International Journal of Oncology 24.2 (2004): 265-272.
Chicago
Pession, Tonelli, Fronza, Sciamanna, Corradini, Sforza, Tedeschi, Marchelli, Montanaro, Camerin, Franzoni, and Guido Paolucci. "Targeted inhibition of NMYC by peptide nucleic acid in N-myc amplified human neuroblastoma cells: cell-cycle inhibition with induction of neuronal cell differentiation and apoptosis." International Journal of Oncology International Journal of Oncology 24 no. 2 (2004): 265-272.