Chromosomal alterations in breast cancer revealed by multicolour fluorescence in situ hybridization

  • Authors: M. B. Watson, H. Bahia, J. N.E. Ashman, H. K. Berrieman, P. Drew, M. J. Lind, J. Greenman, L. Cawkwell
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  • Published online on: Sunday, August 1, 2004
  • Pages: 277-283
  • DOI: 10.3892/ijo.25.2.277

Abstract

The aim of this study was to characterise cytogenetically, breast cancer cell lines and primary tumours to identify chromosomal regions of interest in breast cancer. Multicolour fluorescence in situ hybridization (MFISH) and comparative genomic hybridization (CGH) were used to karyotype five established breast cancer cell lines and two short-term primary tumour cultures. Chromosome 8 was identified as a frequent target for aberrations in all cell lines and one primary culture by MFISH and CGH. CGH identified frequent gains of 1q (all samples) and 14q (all cell lines) and deletion of 22q (all samples). MFISH revealed a t(9;17) translocation in both primary tumours and the T47D cell line. MFISH analysis of the cell lines revealed a significant number of translocations previously unidentified in other studies using similar techniques, highlighting the necessity of utilising data from both primary cultures and established cell lines when investigating complex cytogenetic aberrations using MFISH and CGH.
Journal Cover

August 2004
Volume 25 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.657
Ranked #31/196 Oncology
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APA
Watson, M., Bahia, H., Ashman, J., Berrieman, H., Drew, P., Lind, M., Greenman, J., & Cawkwell, L. (2004). Chromosomal alterations in breast cancer revealed by multicolour fluorescence in situ hybridization. International Journal of Oncology, 25(2), 277-283.
MLA
Watson, Bahia, Ashman, Berrieman, Drew, Lind, Greenman, and L. Cawkwell. "Chromosomal alterations in breast cancer revealed by multicolour fluorescence in situ hybridization." International Journal of Oncology International Journal of Oncology 25.2 (2004): 277-283.
Chicago
Watson, Bahia, Ashman, Berrieman, Drew, Lind, Greenman, and L. Cawkwell. "Chromosomal alterations in breast cancer revealed by multicolour fluorescence in situ hybridization." International Journal of Oncology International Journal of Oncology 25 no. 2 (2004): 277-283.