Effects of WNT/β-catenin pathway activation on signaling through T-cell factor and androgen receptor in prostate cancer cell lines

  • Authors:
    • M. V. Cronauer
    • W. A. Schulz
    • R. Ackermann
    • M. Burchardt
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijo.26.4.1033
  • Pages: 1033-1040
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Dysregulation of the WNT/β-catenin pathway is thought to contribute to prostate cancer progression. Mutations of β-catenin occurring in 5-7% of advanced prostate cancers may act by stimulating TCF-dependent and/or androgen receptor (AR)-dependent transcription. Using a reporter gene approach we found overexpressed mutated β-catenin to enhance AR-regulated probasin-promoter activity in the AR-positive prostate cancer cell line 22Rv1, particularly at low androgen levels. In 22Rv1 cells mutated β-catenin was able to stimulate TCF-dependent transcription but was unable to do so in LNCaP cells where it activates the AR. Since β-catenin mutations are rare in vivo, we studied further possible routes of WNT-pathway modulation. Higher concentrations of LiCl, a GSK3β-inhibitor, were required to activate TCF-dependent rather than AR-dependent reporter constructs. In 22Rv1 overexpression of E-cadherin repressed androgen-dependent transcription, but did not inhibit transcription of TCF-dependent reporter genes as in bladder cancer cell lines. Interestingly, Wnt-3a stimulated proliferation selectively in the AR-positive prostate cancer cell lines 22Rv1 and LNCaP, even though TCF-dependent reporter gene transcription was not induced in LNCaP cells. In summary, the data from our study support the idea that activation of WNT/β-catenin signaling in AR-positive prostate cancer cells may predominantly act through AR-dependent mechanisms rather than classical TCF-dependent mechanisms.

Related Articles

Journal Cover

April 2005
Volume 26 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Cronauer MV, Schulz WA, Ackermann R and Burchardt M: Effects of WNT/β-catenin pathway activation on signaling through T-cell factor and androgen receptor in prostate cancer cell lines. Int J Oncol 26: 1033-1040, 2005
APA
Cronauer, M.V., Schulz, W.A., Ackermann, R., & Burchardt, M. (2005). Effects of WNT/β-catenin pathway activation on signaling through T-cell factor and androgen receptor in prostate cancer cell lines. International Journal of Oncology, 26, 1033-1040. https://doi.org/10.3892/ijo.26.4.1033
MLA
Cronauer, M. V., Schulz, W. A., Ackermann, R., Burchardt, M."Effects of WNT/β-catenin pathway activation on signaling through T-cell factor and androgen receptor in prostate cancer cell lines". International Journal of Oncology 26.4 (2005): 1033-1040.
Chicago
Cronauer, M. V., Schulz, W. A., Ackermann, R., Burchardt, M."Effects of WNT/β-catenin pathway activation on signaling through T-cell factor and androgen receptor in prostate cancer cell lines". International Journal of Oncology 26, no. 4 (2005): 1033-1040. https://doi.org/10.3892/ijo.26.4.1033