Somatostatin effects on the proteome of the LNCaP cell-line

  • Authors:
    • Zhaoxu Liu
    • Sofia Bengtsson
    • Morten Krogh
    • Marcela Marquez
    • Sten Nilsson
    • Peter James
    • Ayodele Aliaya
    • Anders R. Holmberg
  • View Affiliations

  • Published online on: May 1, 2007     https://doi.org/10.3892/ijo.30.5.1173
  • Pages: 1173-1179
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Abstract

Some clinical results indicate that somatostatin (sms) might be useful in the treatment of advanced prostate cancer (HRPC). Because of its transient in vivo half-life only more stable derivatives of sms are of interest in this context. Recent studies have shown that natural sms can be conjugated to a carbohydrate (smsdx) with preservation of sms-like effects on the prostatic tumor cell proteome. The present study identifies some of the affected proteins in an effort to elucidate pathways and proteins that might be of importance for the potential usefulness of sms treatment in HRPC. After incubating the LNCaP cell-line with sms14/smsdx, comparative proteomics was used for analysing and identifying affected proteins. Protein expression patterns were analysed with two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Catalytic mitochondrial and mitochondrial-associated proteins were significantly affected (fold change ≈2 or higher) and they were in general up-regulated. Apoptosis-related proteins were both up-regulated (VDAC1, VDAC2) and down-regulated (PRDX2, TCTP). The fold change was >2 for PRDX2 and <2 for the others. There was a strong agreement between sms and smsdx on the up- and down-regulation of proteins. Sms/smsdx triggered up-regulation of catalytic mitochondrial proteins and seemed to affect apoptosis-related proteins. This could indicate important pathways on which smsdx might be able to curb the progression of HRPC. The results encourage a pending clinical phase II study.

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May 2007
Volume 30 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu Z, Bengtsson S, Krogh M, Marquez M, Nilsson S, James P, Aliaya A and Holmberg AR: Somatostatin effects on the proteome of the LNCaP cell-line. Int J Oncol 30: 1173-1179, 2007
APA
Liu, Z., Bengtsson, S., Krogh, M., Marquez, M., Nilsson, S., James, P. ... Holmberg, A.R. (2007). Somatostatin effects on the proteome of the LNCaP cell-line. International Journal of Oncology, 30, 1173-1179. https://doi.org/10.3892/ijo.30.5.1173
MLA
Liu, Z., Bengtsson, S., Krogh, M., Marquez, M., Nilsson, S., James, P., Aliaya, A., Holmberg, A. R."Somatostatin effects on the proteome of the LNCaP cell-line". International Journal of Oncology 30.5 (2007): 1173-1179.
Chicago
Liu, Z., Bengtsson, S., Krogh, M., Marquez, M., Nilsson, S., James, P., Aliaya, A., Holmberg, A. R."Somatostatin effects on the proteome of the LNCaP cell-line". International Journal of Oncology 30, no. 5 (2007): 1173-1179. https://doi.org/10.3892/ijo.30.5.1173