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Bcr-Abl fusion sequences do not induce immune responses in mice when administered in mouse polyomavirus based virus-like particles

  • Authors:
    • Veronika Hrusková
    • Alena Morávková
    • Katarina Babiarová
    • Viera Ludvíková
    • Jan Fric
    • Vladimír Vonka
    • Jitka Forstová
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/ijo_00000441
  • Pages: 1247-1256
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Abstract

Mouse polyomavirus-like particles (MPyV-VLPs) carrying inside a fragment of the Bcr-Abl hybrid protein containing the epitope of chronic myeloid leukemia fusion region were prepared. A sequence encoding 171 amino acids covering Bcr-Abl breakpoint was fused to the C-terminal part of VP3 minor protein connecting it to the VP1 capsomeres. Chimeric particles, the Bcr-Abl VLPs, were tested for their ability to induce Bcr-Abl specific immune response in mice after their intranasal (i.n.) or intraperitoneal (i.p.) administration without any other adjuvants. Bcr-Abl VLPs induced strong anti-VP1 immune response in both i.n. and i.p. immunized mice. As expected, neither IgG nor IgM anti-Bcr-Abl specific antibodies were detected in the sera of immunized animals. Surprisingly, no specific CTL (cytotoxic T-lymphocyte) activity was proved using two different methods (in vitro cytotoxicity assay with CFSE-labeled target cells and highly sensitive cytotoxicity assay using MHC class I Bcr-Abl specific pentamers). In addition, no proliferative response of T-cells of i.n. immunized mice after in vitro restimulation with antigen-pulsed bone marrow-derived dendritic cells was observed. Taken together, Bcr-Abl breakpoint epitopes appeared to be weak immunogens and even MPyV-VLPs did not provide sufficient adjuvant ability to support induction of immune responses specific to Bcr-Abl fusion zone epitope.

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December 2009
Volume 35 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hrusková V, Morávková A, Babiarová K, Ludvíková V, Fric J, Vonka V and Forstová J: Bcr-Abl fusion sequences do not induce immune responses in mice when administered in mouse polyomavirus based virus-like particles. Int J Oncol 35: 1247-1256, 2009
APA
Hrusková, V., Morávková, A., Babiarová, K., Ludvíková, V., Fric, J., Vonka, V., & Forstová, J. (2009). Bcr-Abl fusion sequences do not induce immune responses in mice when administered in mouse polyomavirus based virus-like particles. International Journal of Oncology, 35, 1247-1256. https://doi.org/10.3892/ijo_00000441
MLA
Hrusková, V., Morávková, A., Babiarová, K., Ludvíková, V., Fric, J., Vonka, V., Forstová, J."Bcr-Abl fusion sequences do not induce immune responses in mice when administered in mouse polyomavirus based virus-like particles". International Journal of Oncology 35.6 (2009): 1247-1256.
Chicago
Hrusková, V., Morávková, A., Babiarová, K., Ludvíková, V., Fric, J., Vonka, V., Forstová, J."Bcr-Abl fusion sequences do not induce immune responses in mice when administered in mouse polyomavirus based virus-like particles". International Journal of Oncology 35, no. 6 (2009): 1247-1256. https://doi.org/10.3892/ijo_00000441