Auto-stimulatory action of secreted caveolin-1 on the proliferation of Ewing's sarcoma cells

  • Authors: Aniruddha Sengupta, Silvia Mateo-Lozano, Oscar M. Tirado, Vicente Notario
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  • Published online on: Thursday, March 3, 2011
  • Pages: 1259-1265
  • DOI: 10.3892/ijo.2011.963

Abstract

Caveolin-1 (CAV1) is highly expressed in Ewing's sarcoma (EWS). We previously showed that increased cellular CAV1 is associated with the regulation of the tumorigenicity, drug resistance and metastatic ability of EWS cells. Because several studies reported that melanoma and prostate cancer cells, which express relatively high CAV1 levels, secrete CAV1, and that secreted CAV1 is associated with tumor progression, our study explored the possibility that EWS cells also secreted CAV1 and that secreted CAV1 may contribute to EWS pathobiology. Results from experiments involving the ectopic expression of a Myc-tagged CAV1 protein in EWS cells as well as the supplementation of culture media with purified CAV1 protein followed by its intracellular localization using immunofluorescence demonstrated that EWS cells secrete CAV1, that they are able to take up the secreted protein, and that extracellular CAV1 enhances EWS cell proliferation. These findings strongly support the notion that secreted CAV1 may also contribute to the malignant properties of EWS.
Journal Cover

May 2011
Volume 38 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Sengupta, A., Mateo-Lozano, S., Tirado, O., & Notario, V. (2011). Auto-stimulatory action of secreted caveolin-1 on the proliferation of Ewing's sarcoma cells. International Journal of Oncology, 38(5), 1259-1265.
MLA
Sengupta, Mateo-Lozano, Tirado, and Vicente Notario. "Auto-stimulatory action of secreted caveolin-1 on the proliferation of Ewing's sarcoma cells." International Journal of Oncology International Journal of Oncology 38.5 (2011): 1259-1265.
Chicago
Sengupta, Mateo-Lozano, Tirado, and Vicente Notario. "Auto-stimulatory action of secreted caveolin-1 on the proliferation of Ewing's sarcoma cells." International Journal of Oncology International Journal of Oncology 38 no. 5 (2011): 1259-1265.