A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis

  • Authors:
    • Ying-Wooi Wan
    • Rebecca A. Raese
    • James E. Fortney
    • Changchang Xiao
    • Dajie Luo
    • John Cavendish
    • Laura F. Gibson
    • Vincent Castranova
    • Yong Qian
    • Nancy Lan Guo
  • View Affiliations

  • Published online on: July 16, 2012     https://doi.org/10.3892/ijo.2012.1556
  • Pages: 1387-1396
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Abstract

Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple signaling pathways was modeled among a set of genes associated with smoking and lung cancer survival. This approach identified and validated a 7-gene signature for lung cancer diagnosis and prognosis in smokers using patient transcriptional profiles (n=847). The smoking-associated gene coexpression networks in lung adenocarcinoma tumors (n=442) were highly significant in terms of biological relevance (network precision = 0.91, FDR<0.01) when evaluated with numerous databases containing multi-level molecular associations. The gene coexpression network in smoking lung adenocarcinoma patients was confirmed in qRT-PCR assays of the identified biomarkers and involved signaling pathway genes in human lung adenocarcinoma cells (H23) treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Furthermore, the western blotting results of p53, phospho‑p53, Rb and EGFR in NNK-treated H23 and transformed normal human lung epithelial cells (BEAS-2B) support their functional involvement in smoking-induced lung cancer carcinogenesis and progression.

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October 2012
Volume 41 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Wan Y, Raese RA, Fortney JE, Xiao C, Luo D, Cavendish J, Gibson LF, Castranova V, Qian Y, Guo NL, Guo NL, et al: A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis. Int J Oncol 41: 1387-1396, 2012
APA
Wan, Y., Raese, R.A., Fortney, J.E., Xiao, C., Luo, D., Cavendish, J. ... Guo, N.L. (2012). A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis. International Journal of Oncology, 41, 1387-1396. https://doi.org/10.3892/ijo.2012.1556
MLA
Wan, Y., Raese, R. A., Fortney, J. E., Xiao, C., Luo, D., Cavendish, J., Gibson, L. F., Castranova, V., Qian, Y., Guo, N. L."A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis". International Journal of Oncology 41.4 (2012): 1387-1396.
Chicago
Wan, Y., Raese, R. A., Fortney, J. E., Xiao, C., Luo, D., Cavendish, J., Gibson, L. F., Castranova, V., Qian, Y., Guo, N. L."A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis". International Journal of Oncology 41, no. 4 (2012): 1387-1396. https://doi.org/10.3892/ijo.2012.1556