Open Access

Expression and clinical significance of CXCR5/CXCL13 in human non‑small cell lung carcinoma

  • Authors:
    • Rajesh Singh
    • Pranav Gupta
    • Goetz H. Kloecker
    • Shailesh Singh
    • James W. Lillard Jr
  • View Affiliations

  • Published online on: September 30, 2014     https://doi.org/10.3892/ijo.2014.2688
  • Pages: 2232-2240
  • Copyright: © Singh et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

CXCR5 and/or CXCL13 expression is elevated in certain carcinomas and lymphomas. To determine if these factors are involved in progression of non‑small cell lung cancer (LuCa), we evaluated their expression in patients with various forms of this disease. Lung biopsies from patients with non‑neoplastic cells (n=8), squamous cell carcinoma (SCC; n=24), or adenocarcinoma (AC; n=54) were stained for CXCR5. Histopathological analysis of these samples showed significantly higher expression of CXCR5 (p<0.001) in carcinomas (i.e., SCCs and ACs) relative to non‑neoplastic lung tissue. Nuclear and membrane CXCR5 intensities were highest in ACs, with median values of 185 and 130, respectively, followed by SCCs with median values of 170 and 110, respectively. The lowest nuclear and membrane expressions of CXCR5 were found in non‑neoplastic tissues, having median values of 142 and 90, respectively. Sera from SCC patients (n=17), AC patients (n=14), and healthy controls (n=9) were tested for the presence of CXCL13. Serum CXCL13 levels in LuCa patients were higher than in healthy controls. CXCR5 expression in cell lines of human non‑small cell lung carcinoma (NCI‑H1915) and small cell lung carcinoma (SW‑1271) were evaluated by flow cytometry. CXCR5 expression was higher in NCI‑H1915 cells relative to SW‑1271 cells. The functional significance of CXCR5 expression was tested in a migration assay. In response to CXCL13, more NCI‑H1915 cells migrated than SW‑1271 cells. These findings suggest that the CXCR5‑CXCL13 axis influences LuCa progression. After validation in larger patient groups, CXCR5 and CXCL13 may prove useful as biomarkers for LuCa. Correspondingly, blockade of this axis could serve as an effective therapy for LuCa.
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December-2014
Volume 45 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Singh R, Gupta P, Kloecker GH, Singh S and Lillard Jr JW: Expression and clinical significance of CXCR5/CXCL13 in human non‑small cell lung carcinoma. Int J Oncol 45: 2232-2240, 2014
APA
Singh, R., Gupta, P., Kloecker, G.H., Singh, S., & Lillard Jr, J.W. (2014). Expression and clinical significance of CXCR5/CXCL13 in human non‑small cell lung carcinoma. International Journal of Oncology, 45, 2232-2240. https://doi.org/10.3892/ijo.2014.2688
MLA
Singh, R., Gupta, P., Kloecker, G. H., Singh, S., Lillard Jr, J. W."Expression and clinical significance of CXCR5/CXCL13 in human non‑small cell lung carcinoma". International Journal of Oncology 45.6 (2014): 2232-2240.
Chicago
Singh, R., Gupta, P., Kloecker, G. H., Singh, S., Lillard Jr, J. W."Expression and clinical significance of CXCR5/CXCL13 in human non‑small cell lung carcinoma". International Journal of Oncology 45, no. 6 (2014): 2232-2240. https://doi.org/10.3892/ijo.2014.2688