miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells

  • Authors:
    • Bin Fu
    • Yibing Wang
    • Xiali Zhang
    • Bin Lang
    • Xiaocheng Zhou
    • Xiaoyuan Xu
    • Tao Zeng
    • Weipeng Liu
    • Xu Zhang
    • Ju Guo
    • Gongxian Wang
  • View Affiliations

  • Published online on: January 13, 2015     https://doi.org/10.3892/ijo.2015.2837
  • Pages: 1169-1180
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Abstract

Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR‑221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR‑221. As a result, the repression of miR‑221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells.
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March-2015
Volume 46 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Fu B, Wang Y, Zhang X, Lang B, Zhou X, Xu X, Zeng T, Liu W, Zhang X, Guo J, Guo J, et al: miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells. Int J Oncol 46: 1169-1180, 2015
APA
Fu, B., Wang, Y., Zhang, X., Lang, B., Zhou, X., Xu, X. ... Wang, G. (2015). miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells. International Journal of Oncology, 46, 1169-1180. https://doi.org/10.3892/ijo.2015.2837
MLA
Fu, B., Wang, Y., Zhang, X., Lang, B., Zhou, X., Xu, X., Zeng, T., Liu, W., Zhang, X., Guo, J., Wang, G."miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells". International Journal of Oncology 46.3 (2015): 1169-1180.
Chicago
Fu, B., Wang, Y., Zhang, X., Lang, B., Zhou, X., Xu, X., Zeng, T., Liu, W., Zhang, X., Guo, J., Wang, G."miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells". International Journal of Oncology 46, no. 3 (2015): 1169-1180. https://doi.org/10.3892/ijo.2015.2837