Open Access

Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs

  • Authors:
    • Alessandro Paolini
    • Valeria Curti
    • Francesca Pasi
    • Giuliano Mazzini
    • Rosanna Nano
    • Enrica Capelli
  • View Affiliations

  • Published online on: February 2, 2015     https://doi.org/10.3892/ijo.2015.2864
  • Pages: 1491-1497
  • Copyright: © Paolini et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Glioblastoma multiforme (GBM) is the most malignant primary brain tumor in adulthood, characterized by very high recurrence. Following the limited results for conventional therapies, novel therapeutic agents are under investigation. Among the putative new molecules, gallic acid (GA) represents a promising new anticancer drug. The anticancer effect of this drug has been based on its antioxidant effects. The aim of the present study was to investigate the toxic effects of GA on the T98G human glioblastoma cell line and its capacity to modulate the expression of microRNAs targeting the genes involved in tumor growth and invasion. Cytotoxicity, clonogenic ability and cell migration after GA treatment were tested. Moreover, the expression of miRNAs that target genes for antioxidant mitochondrial enzymes (mir-17-3p), p-21 protein (mir-21-5p) and ATM (mir-421-5p) was determined by qRT-PCR. The results confirmed in the T98G cells the anti-proliferative effect of GA reported for other glioma cell lines and showed that the miRNA expression changes depending on GA concentrations. Different GA concentrations can determine a protective or a toxic effect on tumor cells. Thus, the key for GA to induce a specific anticancer action is to use an optimal concentration that avoids these twin effects.
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April-2015
Volume 46 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Paolini A, Curti V, Pasi F, Mazzini G, Nano R and Capelli E: Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs. Int J Oncol 46: 1491-1497, 2015
APA
Paolini, A., Curti, V., Pasi, F., Mazzini, G., Nano, R., & Capelli, E. (2015). Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs. International Journal of Oncology, 46, 1491-1497. https://doi.org/10.3892/ijo.2015.2864
MLA
Paolini, A., Curti, V., Pasi, F., Mazzini, G., Nano, R., Capelli, E."Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs". International Journal of Oncology 46.4 (2015): 1491-1497.
Chicago
Paolini, A., Curti, V., Pasi, F., Mazzini, G., Nano, R., Capelli, E."Gallic acid exerts a protective or an anti-proliferative effect on glioma T98G cells via dose-dependent epigenetic regulation mediated by miRNAs". International Journal of Oncology 46, no. 4 (2015): 1491-1497. https://doi.org/10.3892/ijo.2015.2864