A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells

  • Authors:
    • Yukari Nishii
    • Miki Yamaguchi
    • Yasutoshi Kimura
    • Tadashi Hasegawa
    • Hiroyuki Aburatani
    • Hiroaki Uchida
    • Koichi Hirata
    • Yuji Sakuma
  • View Affiliations

  • Published online on: February 5, 2015     https://doi.org/10.3892/ijo.2015.2880
  • Pages: 1781-1787
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Abstract

Pancreatic cancer is one of the most severe forms of malignancy. Patients with unresectable or metastatic pancreatic cancer usually receive chemotherapy that causes various adverse effects. Antibody-drug conjugates (ADCs), drugs developed by conjugating an anticancer agent to a monoclonal antibody (mAb), can alleviate the side effects of chemotherapy because ADCs selectively bind to cancer cells expressing a particular antigen. We recently developed the recombinant protein DT3C comprising diphtheria toxin (DT) lacking the receptor-binding domain but containing the C1, C2, and C3 domains of Streptococcus protein G (3C). The mAb-DT3C conjugates can be used to select mAbs that are internalized by cells, because the conjugates decrease cell viability only when they are internalized by cells through Ab-antigen reactions. We developed a new mAb to be internalized by TCC-PAN2 cells, a pancreatic carcinoma cell line. The mAb, designated TCC56, recognized Mucin 13 (MUC13), while TCC56‑DT3C conjugates induced cell death in TCC-PAN2 cells expressing MUC13. We found that MUC13 was expressed, at least partially, in all 40 pancreatic ductal carcinoma tissues and adjacent non-cancerous tissues analyzed. The expression levels of MUC13 in pancreatic cancer tissues were greater than those in normal tissues. Our findings suggest that MUC13 can be a target molecule for pancreatic cancer treatment. ADCs, including mAb TCC56, could be promising anticancer agents to alleviate the adverse effects of chemotherapy.
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April-2015
Volume 46 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nishii Y, Yamaguchi M, Kimura Y, Hasegawa T, Aburatani H, Uchida H, Hirata K and Sakuma Y: A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells. Int J Oncol 46: 1781-1787, 2015
APA
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H. ... Sakuma, Y. (2015). A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells. International Journal of Oncology, 46, 1781-1787. https://doi.org/10.3892/ijo.2015.2880
MLA
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H., Hirata, K., Sakuma, Y."A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells". International Journal of Oncology 46.4 (2015): 1781-1787.
Chicago
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H., Hirata, K., Sakuma, Y."A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells". International Journal of Oncology 46, no. 4 (2015): 1781-1787. https://doi.org/10.3892/ijo.2015.2880