Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line

  • Authors:
    • Xin Xu
    • Li-Juan Qian
    • Xing-Yun Su
    • Kui-Feng He
    • Ke-Tao Jin
    • Lin-Hui Gu
    • Jian-Guo Feng
    • Guang-Liang Li
    • Quan Zhou
    • Zhen-Zhen Xu
    • Hao-Hao Wang
    • Jing Zhang
    • Jiang Cao
    • Li-Song Teng
  • View Affiliations

  • Published online on: April 16, 2015     https://doi.org/10.3892/ijo.2015.2966
  • Pages: 2479-2487
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Abstract

Signet ring cell gastric cancer (SRCGC) has very poor prognosis worldwide, and studying its molecular characteristics is urgent for improving the outcome. However, few well-characterized SRCGC cell lines are available for research. Therefore, we established a novel cell line GCSR1, from a Chinese male SRCGC patient. Cell morphology of GCSR1 in culture, maintained in vitro for over 90 passages, is similar to the cells from the patient. GCSR1 cells proliferated in vitro with a doubling time of 67.65 h. Karyotyping showed they were aneuploid. Missense mutation occurred in codon 193 of P53 and deletion occurred in exons 1 and 3 of P16. Results of CCK8 assay revealed that GCSR1 was more resistant to 5-fluorouracil (5-FU) and mitomycin (MMC) than other gastric cancer cell lines. Stem cell marker assay by flow cytometry showed that GCSR1 had high proportion of CD44+ and/or CD133+ cells. It formed colonies easily in soft agar and generated xenograft tumors in nude mice. In conclusion, GCSR1 is a well-established, well-characterized multi-drug resistant cell line with abundant cancer stem cells.
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June-2015
Volume 46 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Xu X, Qian L, Su X, He K, Jin K, Gu L, Feng J, Li G, Zhou Q, Xu Z, Xu Z, et al: Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line. Int J Oncol 46: 2479-2487, 2015
APA
Xu, X., Qian, L., Su, X., He, K., Jin, K., Gu, L. ... Teng, L. (2015). Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line. International Journal of Oncology, 46, 2479-2487. https://doi.org/10.3892/ijo.2015.2966
MLA
Xu, X., Qian, L., Su, X., He, K., Jin, K., Gu, L., Feng, J., Li, G., Zhou, Q., Xu, Z., Wang, H., Zhang, J., Cao, J., Teng, L."Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line". International Journal of Oncology 46.6 (2015): 2479-2487.
Chicago
Xu, X., Qian, L., Su, X., He, K., Jin, K., Gu, L., Feng, J., Li, G., Zhou, Q., Xu, Z., Wang, H., Zhang, J., Cao, J., Teng, L."Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line". International Journal of Oncology 46, no. 6 (2015): 2479-2487. https://doi.org/10.3892/ijo.2015.2966