c-Jun-mediated repression and transactivation of fibronectin

  • Authors: Yukio Hayashi, Yuji Shino, Kengo Saito, Hideki Tanzawa, Hiroshi Shirasawa
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  • Published online on: Tuesday, January 1, 2008
  • Pages: 99-103
  • DOI: 10.3892/mmr.1.1.99

Abstract

Fibronectin (FN) is transactivated by human papillomavirus type 16 (HPV16) E6 via the induction of c-Jun-ATF-2 complexes binding to the cyclic AMP response element (CRE) in the FN promoter. The present study analyzed c-Jun regulation of FN gene expression. Northern and immunoblot analyses showed that c-Jun expression was enhanced in HPV16-E6-expressing cells. However, mouse 10T1/2 cell lines overexpressing c-Jun showed an inverse correlation between the expression levels of c-Jun and those of FN. Luciferase assays indicated that the FN promoter was strongly repressed in c-Jun-overexpressing mouse 10T1/2 cells. Deletion and mutation analyses of the FN promoter revealed that repression of the FN promoter by c-Jun depends on the CRE located at -160 relative to the start site of transcription. Supershift assays of CRE-bound complexes from HPV16-E6-expressing and c-Jun-overexpressing cells suggested that the presence of ATF-2 in the complexes binding to CRE was required for the transactivation of the FN gene. Collectively, our study suggests that the FN gene can be either transactivated or repressed by c-Jun depending on the cell context.
Journal Cover

January 2008
Volume 1 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2013 Impact Factor: 1.484
Ranked #59/122 Medicine Research and Experimental
(total number of cites)

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APA
Hayashi, Y., Shino, Y., Saito, K., Tanzawa, H., & Shirasawa, H. (2008). c-Jun-mediated repression and transactivation of fibronectin. Molecular Medicine Reports, 1(1), 99-103.
MLA
Hayashi, Shino, Saito, Tanzawa, and Hiroshi Shirasawa. "c-Jun-mediated repression and transactivation of fibronectin." Molecular Medicine Reports Molecular Medicine Reports 1.1 (2008): 99-103.
Chicago
Hayashi, Shino, Saito, Tanzawa, and Hiroshi Shirasawa. "c-Jun-mediated repression and transactivation of fibronectin." Molecular Medicine Reports Molecular Medicine Reports 1 no. 1 (2008): 99-103.