Esculetin suppresses lipopolysaccharide-induced inflammatory mediators and cytokines by inhibiting nuclear factor-κB translocation in RAW 264.7 macrophages

  • Authors:
    • Su Hyun Hong
    • Hui-Kyung Jeong
    • Min Ho Han
    • Cheol Park
    • Yung Hyun Choi
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  • Published online on: October 8, 2014     https://doi.org/10.3892/mmr.2014.2613
  • Pages: 3241-3246
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Abstract

Although previous studies have demonstrated that the natural coumarin compound esculetin possesses various pharmacological properties, the molecular mechanism of esculetin-mediated anti-inflammatory potential is not fully understood. In this study, we determined the effects of esculetin on lipopolysaccharide (LPS)-induced inflammatory responses of murine RAW 264.7 macrophages. The results indicate that esculetin inhibits LPS-induced nitric oxide and prostaglandin E2 production in a concentration-dependent manner, and inhibits inducible nitric oxide synthase and cyclooxygenase-2 expression in RAW 264.7 cells. Esculetin also significantly suppresses the production of inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β, which was concomitant with a decrease in their expression levels. Furthermore, it was observed that esculetin attenuated the LPS-mediated nuclear factor-kappa B (NF-κB) translocation associated with the blocking of inhibitor of NF-κB (IκB)-α degradation as well as reactive oxygen species (ROS) production, without any significant cytotoxicity. These data suggest that, by blocking NF-κB activation, esculetin suppresses LPS-elicited inflammatory events, and this is mediated, at least in part, by inhibiting the generation of ROS. Collectively, these findings provide mechanistic insights into the anti-inflammatory action of esculetin in macrophages.
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December-2014
Volume 10 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hong SH, Jeong H, Han MH, Park C and Choi YH: Esculetin suppresses lipopolysaccharide-induced inflammatory mediators and cytokines by inhibiting nuclear factor-κB translocation in RAW 264.7 macrophages. Mol Med Rep 10: 3241-3246, 2014
APA
Hong, S.H., Jeong, H., Han, M.H., Park, C., & Choi, Y.H. (2014). Esculetin suppresses lipopolysaccharide-induced inflammatory mediators and cytokines by inhibiting nuclear factor-κB translocation in RAW 264.7 macrophages. Molecular Medicine Reports, 10, 3241-3246. https://doi.org/10.3892/mmr.2014.2613
MLA
Hong, S. H., Jeong, H., Han, M. H., Park, C., Choi, Y. H."Esculetin suppresses lipopolysaccharide-induced inflammatory mediators and cytokines by inhibiting nuclear factor-κB translocation in RAW 264.7 macrophages". Molecular Medicine Reports 10.6 (2014): 3241-3246.
Chicago
Hong, S. H., Jeong, H., Han, M. H., Park, C., Choi, Y. H."Esculetin suppresses lipopolysaccharide-induced inflammatory mediators and cytokines by inhibiting nuclear factor-κB translocation in RAW 264.7 macrophages". Molecular Medicine Reports 10, no. 6 (2014): 3241-3246. https://doi.org/10.3892/mmr.2014.2613