Open Access

Co-expression analysis of differentially expressed genes in hepatitis C virus-induced hepatocellular carcinoma

  • Authors:
    • Qingfeng Song
    • Chang Zhao
    • Shengqiu Ou
    • Zhibin Meng
    • Ping Kang
    • Liwei Fan
    • Feng Qi
    • Yilong Ma
  • View Affiliations

  • Published online on: October 17, 2014     https://doi.org/10.3892/mmr.2014.2695
  • Pages: 21-28
  • Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The aim of the current study was to investigate the molecular mechanisms underlying hepatitis C virus (HCV)‑induced hepatocellular carcinoma (HCC) using the expression profiles of HCV-infected Huh7 cells at different time points. The differentially expressed genes (DEGs) were identified with the Samr package in R software once the data were normalized. Functional and pathway enrichment analysis of the identified DEGs was also performed. Subsequently, MCODE in Cytoscape software was applied to conduct module analysis of the constructed co-expression networks. A total of 1,100 DEGs were identified between the HCV-infected and control samples at 12, 18, 24 and 48 h post-infection. DEGs at 24 and 48 h were involved in the same signaling pathways and biological processes, including sterol biosynthetic processes and tRNA amino-acylation. There were 22 time series genes which were clustered into 3 expression patterns, and the demarcation point of the 2 expression patterns that 401 overlapping DEGs at 24 and 48 h clustered into was 24 h post-infection. tRNA synthesis‑related biological processes emerged at 24 and 48 h. Replication and assembly of HCV in HCV-infected Huh7 cells occurred mainly at 24 h post-infection. In view of this, the screened time series genes have the potential to become candidate target molecules for monitoring, diagnosing and treating HCV-induced HCC.
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January-2015
Volume 11 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Song Q, Zhao C, Ou S, Meng Z, Kang P, Fan L, Qi F and Ma Y: Co-expression analysis of differentially expressed genes in hepatitis C virus-induced hepatocellular carcinoma. Mol Med Rep 11: 21-28, 2015
APA
Song, Q., Zhao, C., Ou, S., Meng, Z., Kang, P., Fan, L. ... Ma, Y. (2015). Co-expression analysis of differentially expressed genes in hepatitis C virus-induced hepatocellular carcinoma. Molecular Medicine Reports, 11, 21-28. https://doi.org/10.3892/mmr.2014.2695
MLA
Song, Q., Zhao, C., Ou, S., Meng, Z., Kang, P., Fan, L., Qi, F., Ma, Y."Co-expression analysis of differentially expressed genes in hepatitis C virus-induced hepatocellular carcinoma". Molecular Medicine Reports 11.1 (2015): 21-28.
Chicago
Song, Q., Zhao, C., Ou, S., Meng, Z., Kang, P., Fan, L., Qi, F., Ma, Y."Co-expression analysis of differentially expressed genes in hepatitis C virus-induced hepatocellular carcinoma". Molecular Medicine Reports 11, no. 1 (2015): 21-28. https://doi.org/10.3892/mmr.2014.2695