MicroRNA‑210 knockdown contributes to apoptosis caused by oxygen glucose deprivation in PC12 cells

  • Authors:
    • Jie Qiu
    • Xiao‑Yu Zhou
    • Xiao‑Guang Zhou
    • Yong Li
    • Rui Cheng
    • Hai‑Ying Liu
  • View Affiliations

  • Published online on: October 15, 2014     https://doi.org/10.3892/mmr.2014.2651
  • Pages: 719-723
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Abstract

It was previously demonstrated that microRNA-210 (miR-210) exhibited neuroprotective effects in a murine model of hypoxic‑ischemic encephalopathy via inhibition of apoptosis. The aim of the present study was to further elucidate the effect of miR-210 on apoptosis in PC12 cells following transfection with miR‑210 inhibitors and exposure to oxygen glucose depri­vation (OGD). The expression levels of miR‑210 were identified using reverse transcription‑quantitative polymerase chain reaction analysis. Apoptosis was investigated using Annexin V‑fluorescein isothiocyanate assays. Apoptosis‑related protein expression levels were studied with western blot analysis. The results showed that the expression levels of miR‑210 were upregulated in PC12 cells following a 4‑h exposure to OGD, relative to those in normoxic control cells. miR‑210 knockdown increased cell apoptosis by inducing caspase activity and regulating the balance between Bcl‑2 and Bax levels. The present study demonstrated that miR‑210 knockdown induced cell apoptosis using an ex vivo model of ischemic hypoxia (IH). Knockdown of miR‑210 represents a potential novel therapeutic approach to combat neonatal IH.
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January-2015
Volume 11 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Qiu J, Zhou XY, Zhou XG, Li Y, Cheng R and Liu HY: MicroRNA‑210 knockdown contributes to apoptosis caused by oxygen glucose deprivation in PC12 cells. Mol Med Rep 11: 719-723, 2015
APA
Qiu, J., Zhou, X., Zhou, X., Li, Y., Cheng, R., & Liu, H. (2015). MicroRNA‑210 knockdown contributes to apoptosis caused by oxygen glucose deprivation in PC12 cells. Molecular Medicine Reports, 11, 719-723. https://doi.org/10.3892/mmr.2014.2651
MLA
Qiu, J., Zhou, X., Zhou, X., Li, Y., Cheng, R., Liu, H."MicroRNA‑210 knockdown contributes to apoptosis caused by oxygen glucose deprivation in PC12 cells". Molecular Medicine Reports 11.1 (2015): 719-723.
Chicago
Qiu, J., Zhou, X., Zhou, X., Li, Y., Cheng, R., Liu, H."MicroRNA‑210 knockdown contributes to apoptosis caused by oxygen glucose deprivation in PC12 cells". Molecular Medicine Reports 11, no. 1 (2015): 719-723. https://doi.org/10.3892/mmr.2014.2651