Open Access

Lymphotoxin β receptor activation promotes bladder cancer in a nuclear factor-κB-dependent manner

  • Authors:
    • Mo Shen
    • Xiuzhi Duan
    • Ping Zhou
    • Wu Zhou
    • Xiuling Wu
    • Siqi Xu
    • Yuhua Chen
    • Zhihua Tao
  • View Affiliations

  • Published online on: October 30, 2014     https://doi.org/10.3892/mmr.2014.2826
  • Pages: 783-790
  • Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Bladder cancer (BCa) is the most common tumor of the urinary system. Chronic inflammation in the papillary urothelial neoplasm of low malignant potential (PUNLMP)may contribute to carcinogenesis, including that of BCa, via poorly understood mechanisms. In this study, we show that the lymphotoxin β receptor (LTβR) is upregulated in BCa via activation of the canonical and non-canonical nuclear factor-κB (NF-κB) pathways. The mRNA expression of LTβR in 81 BCa, 10 chronic cystitis and 23 healthy bladder mucosa tissues was investigated by reverse transcription-fluorescent quantitative polymerase chain reaction (RT-FQ-PCR), and protein expression was studied in 73 BCa, 30 cystitis and 15 healthy paraffin-embedded tissue sections by immunohistochemistry. Both LTβR mRNA and protein were upregulated in BCa and cystitis compared to the healthy group (P<0.05). The mRNA level of the downstream NF-κB canonical pathway p65 gene and of the non-canonical pathway RelB gene were higher in the BCa and cystitis groups compared to the healthy one. The level of phosphorylated p65 (p-p65) protein of the canonical NF-κB pathway and that of p52, a protein of the non-canonical NF-κB pathway, were also higher in the BCa and cystitis group compared to the healthy group. The levels of these proteins significantly correlated to the pathological grade, clinical stage and lymph node metastasis of BCa patients (P<0.05). In addition, there was a positive correlation between LTβR and NF-κB pathway proteins. Thus, LTβR signaling may be involved in promoting BCa through the NF-κB pathway, and which may represent the molecular link between inflammation and BCa.

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February-2015
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Spandidos Publications style
Shen M, Duan X, Zhou P, Zhou W, Wu X, Xu S, Chen Y and Tao Z: Lymphotoxin β receptor activation promotes bladder cancer in a nuclear factor-κB-dependent manner. Mol Med Rep 11: 783-790, 2015
APA
Shen, M., Duan, X., Zhou, P., Zhou, W., Wu, X., Xu, S. ... Tao, Z. (2015). Lymphotoxin β receptor activation promotes bladder cancer in a nuclear factor-κB-dependent manner. Molecular Medicine Reports, 11, 783-790. https://doi.org/10.3892/mmr.2014.2826
MLA
Shen, M., Duan, X., Zhou, P., Zhou, W., Wu, X., Xu, S., Chen, Y., Tao, Z."Lymphotoxin β receptor activation promotes bladder cancer in a nuclear factor-κB-dependent manner". Molecular Medicine Reports 11.2 (2015): 783-790.
Chicago
Shen, M., Duan, X., Zhou, P., Zhou, W., Wu, X., Xu, S., Chen, Y., Tao, Z."Lymphotoxin β receptor activation promotes bladder cancer in a nuclear factor-κB-dependent manner". Molecular Medicine Reports 11, no. 2 (2015): 783-790. https://doi.org/10.3892/mmr.2014.2826