Open Access

Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells

  • Authors:
    • Xiaomin Yan
    • Xiao-Xue Ke
    • Hailong Zhao
    • Mengying Huang
    • Renjian Hu
    • Hongjuan Cui
  • View Affiliations

  • Published online on: October 29, 2014     https://doi.org/10.3892/mmr.2014.2814
  • Pages: 791-796
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Triptolide is a diterpene triepoxide, extracted from the Chinese herb Tripterygium wilfordii Hook F, which has been shown to have antitumor activity in a number of cancers. Neuroblastoma is an aggressive extracranial pediatric solid tumor, with significant chemotherapeutic resistance. In this study, triptolide was hypothesized to be a potential therapeutic agent for neuroblastoma. The effects of triptolide on neuroblastoma cell growth and tumor development were investigated. Cell growth and proliferation were evaluated using a cell counting kit‑8 assay and a 5-bromo-2-deoxyuridine staining assay. Cell cycle and apoptosis were detected by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9. The tumorigenicity of neuroblastoma cells was assessed by a soft agar clonogenic assay and an in vivo tumorigenic assay. The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase. A soft agar assay indicated that triptolide inhibited the colony‑forming ability of BE(2)‑C neuroblastoma cells. The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo. The data suggested that this Chinese herb may be a potential novel chemotherapeutic agent for neuroblastoma.
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February-2015
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Spandidos Publications style
Yan X, Ke X, Zhao H, Huang M, Hu R and Cui H: Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells. Mol Med Rep 11: 791-796, 2015
APA
Yan, X., Ke, X., Zhao, H., Huang, M., Hu, R., & Cui, H. (2015). Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells. Molecular Medicine Reports, 11, 791-796. https://doi.org/10.3892/mmr.2014.2814
MLA
Yan, X., Ke, X., Zhao, H., Huang, M., Hu, R., Cui, H."Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells". Molecular Medicine Reports 11.2 (2015): 791-796.
Chicago
Yan, X., Ke, X., Zhao, H., Huang, M., Hu, R., Cui, H."Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells". Molecular Medicine Reports 11, no. 2 (2015): 791-796. https://doi.org/10.3892/mmr.2014.2814