Open Access

Effects of Treg cells and IDO on human epithelial ovarian cancer cells under hypoxic conditions

  • Authors:
    • Jun Liu
    • Haiyan Zhang
    • Luoqi Jia
    • Hong Sun
  • View Affiliations

  • Published online on: November 7, 2014     https://doi.org/10.3892/mmr.2014.2893
  • Pages: 1708-1714
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The aim of the present study was to explore the effect of hypoxia on ovarian cancer. A total of 6 samples were analyzed: SKOV3‑IP cells (ovarian cancer cell line); SKOV3‑IP and regulatory T (Treg) cells; SKOV3‑IP and cytotoxic T lymphocytes (CTLs); SKOV3‑IP and natural killer (NK) cells; SKOV3‑IP co-cultured with CTLs and Treg cells; and SKOV3‑IP co-cultured with Treg cells and NK cells. The expression of indoleamine 2,3‑dioxygenase (IDO) was detected by reverse transcription-polymerase chain reaction (RT‑PCR) and western blot analysis. An enzyme‑linked immunosorbent assay (ELISA) was used to detect the concentration of transforming growth factor‑β (TGF‑β), interferon‑γ (IFN‑γ), interleukin‑2 (IL‑2), interleukin‑10 (IL‑10), and perforin. Moreover, ovarian cancer cell apoptosis and invasive ability were examined using flow cytometry and a Transwell chamber assay. IDO expression was significantly reduced in hypoxia and enhanced by Treg cells. Treg cells inhibited the IL‑2, IFN‑γ and perforin secretion, and significantly (P<0.05) increased the IL‑10 and TGF‑β levels. The effects of Treg cells were enhanced with prolongation of the cell exposure to hypoxic conditions. In addition, Treg cells attenuated the promotive effect of CTLs and NK cells on cancer cell apoptosis. In addition, Treg cells significantly increased the SKOV3‑IP invasive ability (P=0.00109) under hypoxic conditions. Our results suggest that IDO and Treg cells may serve as important therapeutic targets for patients with ovarian cancer.
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March-2015
Volume 11 Issue 3

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Spandidos Publications style
Liu J, Zhang H, Jia L and Sun H: Effects of Treg cells and IDO on human epithelial ovarian cancer cells under hypoxic conditions. Mol Med Rep 11: 1708-1714, 2015
APA
Liu, J., Zhang, H., Jia, L., & Sun, H. (2015). Effects of Treg cells and IDO on human epithelial ovarian cancer cells under hypoxic conditions. Molecular Medicine Reports, 11, 1708-1714. https://doi.org/10.3892/mmr.2014.2893
MLA
Liu, J., Zhang, H., Jia, L., Sun, H."Effects of Treg cells and IDO on human epithelial ovarian cancer cells under hypoxic conditions". Molecular Medicine Reports 11.3 (2015): 1708-1714.
Chicago
Liu, J., Zhang, H., Jia, L., Sun, H."Effects of Treg cells and IDO on human epithelial ovarian cancer cells under hypoxic conditions". Molecular Medicine Reports 11, no. 3 (2015): 1708-1714. https://doi.org/10.3892/mmr.2014.2893