Open Access

MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9

  • Authors:
    • Tong Han
    • Xiao‑Ping Yi
    • Bo Liu
    • Mu‑Jing Ke
    • Yi‑Xiong Li
  • View Affiliations

  • Published online on: February 3, 2015     https://doi.org/10.3892/mmr.2015.3294
  • Pages: 4115-4120
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

MicroRNAs (miRNAs) represent a class of small non‑coding RNAs regulating gene expression by inducing the degradation of RNA or interfering with translation. Aberrant miRNA expression has been described in several types of cancer in humans. In the present study, it was demonstrated that miR‑145 is downregulated in pancreatic cancer tissues and the Panc‑1 cell line. Restoration of miR‑145 inhibited cell proliferation, invasion and migration in Panc‑1 cells. Neural precursor cell expressed, developmentally down‑regulated 9 (NEDD9) has been identified as a novel potential miR‑145 target using bioinformatics. Using luciferase reporter constructs, it was observed that the NEDD9 3'‑untranslated region is the location of the direct binding site for miR‑145. Additionally, it was identified that miR‑145 is inversely correlated with NEDD9 expression in pancreatic cancer tissues and that restoration of miR‑145 in Panc‑1 cells reduced NEDD9 mRNA and protein expression accompanied by inhibition of cell proliferation, invasion and migration. In conclusion, these findings indicate that miR‑145 may be an effective target for pancreatic cancer therapy.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Han T, Yi XP, Liu B, Ke MJ and Li YX: MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9. Mol Med Rep 11: 4115-4120, 2015
APA
Han, T., Yi, X., Liu, B., Ke, M., & Li, Y. (2015). MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9. Molecular Medicine Reports, 11, 4115-4120. https://doi.org/10.3892/mmr.2015.3294
MLA
Han, T., Yi, X., Liu, B., Ke, M., Li, Y."MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9". Molecular Medicine Reports 11.6 (2015): 4115-4120.
Chicago
Han, T., Yi, X., Liu, B., Ke, M., Li, Y."MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9". Molecular Medicine Reports 11, no. 6 (2015): 4115-4120. https://doi.org/10.3892/mmr.2015.3294