Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice

  • Authors:
    • Xiaojuan Zhang
    • Zhenggang Luan
    • Yingjian Liang
    • Yina Liu
    • Xiaochun Ma
  • View Affiliations

  • Published online on: January 26, 2015     https://doi.org/10.3892/mmr.2015.3251
  • Pages: 4513-4517
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Abstract

High mobility group box 1 (HMGB1) is a systemic inflammation‑associated cytokine mediator. The aim of the present study was to examine the effect of the downregulation of HMGB1 in a lipopolysaccharide (LPS)‑induced mouse model of acute lung injury (ALI). It was identified that serum levels of tumor necrosis factor‑α and interleukin‑1β, lung myeloperoxidase activity and malondialdehyde content, as well as lung wet/dry weight ratios were all increased following LPS challenge. However, LPS‑mediated increases in these parameters were significantly downregulated in HMGB1 small interfering (si)RNA‑treated mice versus the negative control siRNA‑treated mice. In addition, the administration of HMGB1 siRNA in LPS‑treated mice resulted in a decreased DNA binding activity of nuclear factor‑κB (NF‑κB) in the lung. It was demonstrated that downregulation of HMGB1 decreases inflammation and the severity of sepsis associated with ALI, possibly via inhibiting the NF‑κB DNA‑binding activity. The present data support HMGB1 as a contributor to the pathogenesis of LPS‑induced sepsis and ALI.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang X, Luan Z, Liang Y, Liu Y and Ma X: Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice. Mol Med Rep 11: 4513-4517, 2015
APA
Zhang, X., Luan, Z., Liang, Y., Liu, Y., & Ma, X. (2015). Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice. Molecular Medicine Reports, 11, 4513-4517. https://doi.org/10.3892/mmr.2015.3251
MLA
Zhang, X., Luan, Z., Liang, Y., Liu, Y., Ma, X."Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice". Molecular Medicine Reports 11.6 (2015): 4513-4517.
Chicago
Zhang, X., Luan, Z., Liang, Y., Liu, Y., Ma, X."Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice". Molecular Medicine Reports 11, no. 6 (2015): 4513-4517. https://doi.org/10.3892/mmr.2015.3251