microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA

  • Authors:
    • Man Li
    • Shi‑Ming Chen
    • Chen Chen
    • Zi‑Xiong Zhang
    • Meng‑Yuan Dai
    • Lei‑Bo Zhang
    • Shui‑Bin Wang
    • Qiong Dai
    • Ze‑Zhang Tao
  • View Affiliations

  • Published online on: January 30, 2015     https://doi.org/10.3892/mmr.2015.3287
  • Pages: 4645-4649
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Abstract

Aberrant microRNA (miRNA) expression has been linked to cancer development. In this study, we aimed to investigate whether the anti‑cancer effect of miRNA‑299‑3p on laryngeal cancer Hep‑2 cells is mediated through targeting human telomerase reverse transcriptase (hTERT). The expression of miR‑299‑3p in laryngeal cancer Hep‑2 cells and human osteosarcoma U2OS cells was quantified by stem‑loop‑mediated reverse transcription quantitative polymerase chain reaction. miR‑299‑3p mimic was transfected into Hep‑2 cells to induce overexpression of miR‑299‑3p. A CCK‑8 assay was performed to identify the effects of miR‑299‑3p overexpression on the proliferation of Hep‑2 cells. Western blot analysis was carried out to determine the expression of hTERT protein. A significant decrease was noted in the expression of miR‑299‑3p in Hep‑2 cells compared with that of U2OS cells (P<0.05). Overexpression of miR‑299‑3p resulted in a notable inhibition of cellular proliferation (P<0.05), as well as downregulation of hTERT mRNA and protein in Hep‑2 cells (P>0.05). The expression of miR‑299‑3p is downregulated in human laryngeal cancer Hep‑2 cells. Overexpression of miR‑299‑3p inhibits Hep‑2 cell growth by targeting the 3'‑untranslated region of hTERT mRNA.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li M, Chen SM, Chen C, Zhang ZX, Dai MY, Zhang LB, Wang SB, Dai Q and Tao ZZ: microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA. Mol Med Rep 11: 4645-4649, 2015
APA
Li, M., Chen, S., Chen, C., Zhang, Z., Dai, M., Zhang, L. ... Tao, Z. (2015). microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA. Molecular Medicine Reports, 11, 4645-4649. https://doi.org/10.3892/mmr.2015.3287
MLA
Li, M., Chen, S., Chen, C., Zhang, Z., Dai, M., Zhang, L., Wang, S., Dai, Q., Tao, Z."microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA". Molecular Medicine Reports 11.6 (2015): 4645-4649.
Chicago
Li, M., Chen, S., Chen, C., Zhang, Z., Dai, M., Zhang, L., Wang, S., Dai, Q., Tao, Z."microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA". Molecular Medicine Reports 11, no. 6 (2015): 4645-4649. https://doi.org/10.3892/mmr.2015.3287