Open Access

Thymosin β4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition

  • Authors:
    • Xiaojun Fu
    • Peiyuan Cui
    • Fangfang Chen
    • Jianzhong Xu
    • Li  Gong
    • Lei Jiang
    • Dakun Zhang
    • Yongtao Xiao
  • View Affiliations

  • Published online on: February 16, 2015     https://doi.org/10.3892/mmr.2015.3359
  • Pages: 127-132
  • Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Hepatoblastoma (HB) is the most common malignant hepatic tumor in children and complete surgical resection offers the highest possibility for cure in this disease. Tumor metastasis is the principle obstacle to the development of efficient treatments for patients with HB. The present study aimed to measure the expression levels of thymosin β4 (Tβ4) in liver samples from patients with HB and to investigate the involvement of Tβ4 in HB metastasis. The expression of Tβ4 was significantly higher in liver samples from patients with metastatic HB and in the HepG2 metastatic HB cell line, compared with that in adjacent healthy liver samples and in the L02 healthy hepatic cell line. By contrast, the expression levels of epithelial-cadherin (E-cadherin) and cytosolic accumulation of β-catenin, the two most prominent markers involved in epithelial‑mesenchymal transition (EMT), were reduced in liver specimens from patients with metastatic HB compared with that of healthy adjacent control tissue. HepG2 cells were transfected with small interfering‑RNA in order to downregulate Tβ4 gene expression. This resulted in a reduced cell migratory capacity compared with control cells. Tβ4 gene expression knockdown significantly inhibited transforming growth factor β1‑mediated‑EMT in vitro by upregulating the expression of E‑cadherin. The results of the present study suggested that Tβ4 may promote HB metastasis via the induction of EMT, and that Tβ4 may therefore be a target for the development of novel treatments for patients with HB.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Fu X, Cui P, Chen F, Xu J, Gong L, Jiang L, Zhang D and Xiao Y: Thymosin β4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12: 127-132, 2015
APA
Fu, X., Cui, P., Chen, F., Xu, J., Gong, L., Jiang, L. ... Xiao, Y. (2015). Thymosin β4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Molecular Medicine Reports, 12, 127-132. https://doi.org/10.3892/mmr.2015.3359
MLA
Fu, X., Cui, P., Chen, F., Xu, J., Gong, L., Jiang, L., Zhang, D., Xiao, Y."Thymosin β4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition". Molecular Medicine Reports 12.1 (2015): 127-132.
Chicago
Fu, X., Cui, P., Chen, F., Xu, J., Gong, L., Jiang, L., Zhang, D., Xiao, Y."Thymosin β4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition". Molecular Medicine Reports 12, no. 1 (2015): 127-132. https://doi.org/10.3892/mmr.2015.3359