Identification of active ingredients in Wuzhuyu decoction improving migraine in mice by spectral efficiency association

  • Authors:
    • Xueqiang Pan
    • Manyuan Wang
    • Yanchuan Wu
    • Xuran Lu
    • Yawen Shang
    • Yongsong Xu
    • Yongsong Zhai
    • Jing Li
    • Zhaoxia Li
    • Muxin Gong
  • View Affiliations

  • Published online on: March 18, 2015     https://doi.org/10.3892/mmr.2015.3506
  • Pages: 1524-1534
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Wuzhuyu decoction is a traditional Chinese medicine used for the effective treatment of migraines, termed ‘Jueyin headache’, in China. However, there have been few investigations to clarify the composition of Wuzhuyu decoction for the treatment of migraines. In the present study, 10 types of Wuzhuyu decoction were analyzed by chromatograms. 5‑hydroxytryptamine (5‑HT)-depletion mouse models of migraine were prepared by subcutaneous injection of reserpine and placement of autologous blood clots in the cerebral cortex. The levels of 5‑HT, noradrenaline (NE), dopamine (DA), nitric oxide (NO) and nitric oxide synthase (NOS) in the brain tissues and sera of the mice were determined. The ingredients and pharmacodynamic indices of the Wuzhuyu decoctions were analyzed using spectral efficiency association by partial least squares regression. The levels of 5‑HT, NE and DA in the mouse brain tissues were reduced to 337.785±84.504, 171.173±65.172 and 242.075±158.621 mg/g brain tissue, respectively. The level of NO in the brain tissues increased to 0.425±0.184 µmol/g protein and the activities of NOS in the brain tissues and sera increased to 0.719±0.477 U/mg and 50.688±8.132 U/ml, respectively. Regarding the ingredients of the Wuzhuyu decoction, those with significant regression coefficients were ginsenoside‑Rg1, Re, Rb1, rutaevine (Rv), limonin (Li), evodiamine (Ev), rutaecarpine (Ru) and substance X (awaiting identification). Rg1, Re, Rb1, Rv, Li, Ev, Ru and X in the Wuzhuyu decoction were observed to yield the pharmacological effects, whereas Rb1, Rv and Ev were important in index improvement.
View Figures
View References

Related Articles

Journal Cover

July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Pan X, Wang M, Wu Y, Lu X, Shang Y, Xu Y, Zhai Y, Li J, Li Z, Gong M, Gong M, et al: Identification of active ingredients in Wuzhuyu decoction improving migraine in mice by spectral efficiency association. Mol Med Rep 12: 1524-1534, 2015
APA
Pan, X., Wang, M., Wu, Y., Lu, X., Shang, Y., Xu, Y. ... Gong, M. (2015). Identification of active ingredients in Wuzhuyu decoction improving migraine in mice by spectral efficiency association. Molecular Medicine Reports, 12, 1524-1534. https://doi.org/10.3892/mmr.2015.3506
MLA
Pan, X., Wang, M., Wu, Y., Lu, X., Shang, Y., Xu, Y., Zhai, Y., Li, J., Li, Z., Gong, M."Identification of active ingredients in Wuzhuyu decoction improving migraine in mice by spectral efficiency association". Molecular Medicine Reports 12.1 (2015): 1524-1534.
Chicago
Pan, X., Wang, M., Wu, Y., Lu, X., Shang, Y., Xu, Y., Zhai, Y., Li, J., Li, Z., Gong, M."Identification of active ingredients in Wuzhuyu decoction improving migraine in mice by spectral efficiency association". Molecular Medicine Reports 12, no. 1 (2015): 1524-1534. https://doi.org/10.3892/mmr.2015.3506